Product Description

Caspase 3 precursor Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65606-50, BS65606-100

Swiss-Prot: P55213

Host: Rabbit

Caspase 3 precursor Polyclonal Antibody-BS65606

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, (predicted: Dog, Rabbit, )

Applications: WB, ，

All Applications: WB=1:500-2000 IHC-P=1:100-500

Background: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Purification and Purity: affinity purified by Protein A

Specificity: Caspase 3 Polyclonal Antibody detects endogenous levels of Caspase 3 protein.

Bioworld Molecular Weight: 28kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Caspase 3 precursor at 1/1000 dilution, Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Primary: Anti-Caspase 3 precursor at 1/1000 dilution

Alternative Name: Caspase 3 precursor; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.

Immunogen: KLH conjugated synthetic peptide derived from human Caspase 3 precursor:11-120/277

Modification: Unmodified

Conjugate: Unconjugated