Caspase-6 Polyclonal Antibody-BS90188

Caspase-6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90188-50, BS90188-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55212(Human) O08738(Mouse) O35397(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/ caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose)polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Caspase-6 polyclonal antibody detects endogenous levels of Caspase-6 protein.

Bioworld Molecular Weight: 11/33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Caspase-6 on different lysates using anti-Caspase-6 antibody at 1/1,000 dilution.
Positive control: Lane 1: Mouse colon Lane 2: MCF-7, ICC staining Caspase-6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CASP-6, CASP6, Apoptotic protease Mch-2, Apoptotic protease MCH2, CASP-6, CASP6, CASP6, Caspase 6, Caspase 6 apoptosis related cysteine protease, Caspase 6, apoptosis related cysteine peptidase, Caspase-6 subunit p11, Mch2, OTTHUMP00000162742, OTTHUMP00000162743,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified