Product Description

Caspase-9 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90192-50, BS90192-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55211(Human) Q8C3Q9(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Caspase-9 polyclonal antibody detects endogenous levels of Caspase-9 protein.

Bioworld Molecular Weight: 46/35 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Caspase-9 on different lysates using anti-Caspase-9 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: C2C12, ICC staining Caspase-9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CASP-9, APAF-3, ICE-LAP6, CASP9, APAF-3, APAF3, Apoptosis related cysteine peptidase, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, CASP9, Caspase 9 apoptosis related cysteine peptidase, Caspase 9 Dominant Negative, Caspase 9c, Caspase-9, Caspase-9 subunit p10, ICE LAP6, ICE like apoptotic protease 6, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, PPP1R56, protein phosphatase 1, regulatory subunit 56, RNCASP9,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified