Product Description

Caspase-9 (T125) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0580-50, AP0580-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimμlation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resμlting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IP

Alternate Names: CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, Caspase-9 subunit p35, Caspase-9 subunit p10

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to Human Caspase-9.

Dilution: WB: 1:1000~1:2000
IP: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Caspase-9 (T125) polyclonal antibody detects endogenous levels of Caspase-9 protein.

Molecular Weight: ~ 37, 40/40, 46 kDa

Pathway: Regμlation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P55211

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only