Product Description

Cathepsin B Polyclonal Antibody

Catalogue Numbers: BS67486-50, BS67486-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P07858

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Cathepsin B (CSTB), part of the papain family of proteases, is a widely expressed lysosomal cysteine endopeptidase . Cathepsin B is produced from a larger precursor form, pro-cathepsin B, which runs at approximately 44 kDa on SDS-PAGE, and is proteolytically processed and glycosylated to form a mature two-chain protein containing a heavy chain (running at 27 and 24 kDa) and a light chain (5 kDa). High levels of cathepsin B are found in macrophages and osteoclasts, as well as various types of cancer cells, including lung, colon, prostate, breast, and stomach. In addition, expression of cathepsin B has been associated with multiple sclerosis , rheumatoid arthritis , and pancreatitis . While generally localized to lysosomes, in cancer alterations can lead to its secretion . Its role in tumor progression is thought to involve promotion of basement membrane degradation, invasion and metastasis. Expression can correlate with poor prognosis for a variety of forms of cancer .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Cathepsin B protein.

Extra Notes: Western blot analysis of Cathepsin B expression in mouse embryo (A), rat lung (B), HCT116 (C), A549 (D) whole cell lysates.

Bioworld Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: CPSB; Cathepsin B; APP secretase; APPS; Cathepsin B1

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cathepsin B. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified