Product Description

Cathepsin LVKH Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90202-50, BS90202-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07711(Human) O60911(Human) P43235(Human) P09668(Human)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:100
IHC:1:50-1:200
FC:1:50-1:100

Background: The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin L (also designated major excreted protein, MEP or CATL) is a member of the peptidase C1 family and has been identified as a protein that is most closely related to cathepsin H. It is a lysosomal cysteine proteinase that mediates intracellular protein catabolism for collagen, elastin and ?-1 protease inhibitor. Cathepsin L is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. Transformed mouse fibroblasts stimulated by growth factors or tumor promoters secrete a form of cathepsin L.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cathepsin LVKH polyclonal antibody detects endogenous levels of Cathepsin LVKH protein.

Bioworld Molecular Weight: 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: A549, ICC staining Cathepsin L/V/K/H in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MEP, CTSL,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified