Product Description

Cathepsin W Polyclonal Antibody

Catalogue Numbers: BS62560-50, BS62560-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P56202

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: Cathepsin W (lymphopain) and cathepsin F comprise a novel subgroup of cathepsin proteases, and are phylogenetically distinct from other human cathepsins. The cathepsin W gene maps to chromosome 11q13.1 and contains ten exons with introns ranging from 81-119 bp. Cathepsin W protein is expressed specifically in CD8+ T-lymphocyte. The expression of cathepsin W first occurs during the differentiation of thyrocytes to CD8+ T-lymphocytes, just as the thymocytes cease expression of CD4+ receptors. In transfected Cos-7 and Hela cells, cathepsin W localizes within the rough endoplasmic reticulum. Cathepsin W contains a unique 21-amino acid peptide insertion between the active site histidine and asparagine residues, in addition to a distictive 8-amino acid carboxy- terminal extension. An extended loop structure in the second or β-sheet domain and an additional disulfide bind are two of several signature features of cathepsin W. Other features of cathepsin W include an additional cysteine, an S2 pocket and an additional residue. Cathepsin W may exist as a dimer with each monomer forming a disulfide bond.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cathepsin W polyclonal antibody detects endogenous levels of Cathepsin W protein.

Extra Notes: Western blot (WB) analysis of Cathepsin W polyclonal antibody at 1:500 dilution
LaneA:RAW264.7 whole cell lysate

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cathepsin W; Lymphopain; CTSW

Immunogen: Synthetic peptide, corresponding to Human Cathepsin W.

Conjugate: Unconjugated

Modification: Unmodified