Cattle TGFb1 (Transforming Growth Factor Beta 1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK5467-48, ELK5467-96, ELK5467-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
Assay Type: Competitive Inhibition
Sensitivity: 4.8 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, platelet-poor plasma, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Cytokine; Tumor immunity; Infection immunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P18341
Research Use Only