CB1 (F189) Polyclonal Antibody-BS2712

CB1 (F189) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2712-50, BS2712-100

Sizes: 50µl, 100µl

Swiss-Prot: P21554

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The cannabinoid receptors (CB1 and CB2) are G-protein coupled receptors that inhibit adenylate cyclase activity in response to psychoactive cannabinoids. CB1 is expressed in brain tissue and, in low levels, in testis. Phosporylation of CB1 on serine 316 leads to a disruption of CB1-mediated inhibition of calcium channels and activation of potassium currents. This response system is thought to be involved in specific brain functions, such as nociception, control of movement, memory, and neuroendocrine regulation as well as having a possible role in brain development. In addition, CB1 may mediate the addictive behavior involved with the use of psychoactive cannabinoids, such as THC in marijuana.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CB1 (F189) polyclonal antibody detects endogenous levels of CB1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CB1 (F189) pAb at 1:500 dilution
Lane 1: BV2 whole cell lysate (40ug)
Lane 2: The Brain tissue lysate of Rat (40ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: U-87MG whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of CB1 (F189) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 53 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cannabinoid receptor 1; CB-R; CB1; CANN6; CNR1; CNR

Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human CB1.

Conjugate: Unconjugated

Modification: Unmodified