Product Description

CBLB Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-68339-60, E-AB-68339-120, E-AB-68339-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: CBLB

Target Synonym: CBLB; Cbl-b; Nbla00127; RNF56

Research Areas: Cancer, Cell Biology, Immunology

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q13191

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor, BCR (B-cell receptor and FCER1 (high affinity immunoglobulin epsilon receptor signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA signaling pathway via ubiquitination and internalization of PDGFRA (By similarity.

Concentration: 1 mg/mL

Immunogen: Recombinant fusion protein of human CBLB

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 86kDa/90kDa/109kDa

Observed MW: 109kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only