Product Description

CBP80 (R5) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2040-50, BS2040-100

Sizes: 50µl, 100µl

Swiss-Prot: Q09161

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: In eukaryotes, the majority of mRNAs have an m (7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex(CBC). CBC consists of two cap binding proteins CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CBP80 (R5) polyclonal antibody detects endogenous levels of CBP80 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CBP80 (R5) pAb at 1:500 dilution
Lane 1: K562 whole cell lysate (10ug)
Lane 2: Hela whole cell lysate (20ug)
Lane 3: The Brain tissue lysate of Mouse (40ug)
Lane 4: The Brain tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of CBP80 (R5) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 88 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Nuclear cap-binding protein subunit 1; 80 kDa nuclear cap-binding protein; CBP80; NCBP 80 kDa subunit; NCBP1; CBP80; NCBP

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human CBP80.

Conjugate: Unconjugated

Modification: Unmodified