Product Description

CD100 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66385-50, MB66385-100

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: Q92854

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background: Semaphorin-4D/CD100 (Sema4D) is a disulfide-linked homodimeric type 1 transmembrane glycoprotein belonging to the class IV family of membrane bound semaphorins. The extracellular domain of Sema4D contains a cysteine-rich semaphorin-like domain, an Ig-like domain, and a PSI domain . Research studies have suggested that the cytoplasmic domain has a signaling function as it is phosphorylated on serine residues . Initial studies involving Sema4D revealed that it was implicated in axon guidance within the central nervous system through binding its high affinity receptor, plexin-B1 . Sema4D function has also been extensively characterized in the immune system and is the first semaphorin found to be expressed on the surface of many types of immune cells . In the immune system, CD72 serves as a low-affinity receptor for Sema4D and research studies have shown that Sema4D not only regulates T-cell activation but is also involved in the regulation of B-cell survival and differentiation . Many of the physiologic effects of Sema4D in the immune system are regulated by a soluble extracellular domain-containing fragment generated through proteolytic cleavage .Sema4D has also been implicated in oncogenesis as research studies have demonstrated overexpression in multiple types of solid tumors . The role of Sema4D in oncogenesis, in part, has been linked to its ability to promote tumor angiogenesis , cell invasion , and immunosuppression through enhancement of myeloid derived suppressor cell function .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD100 protein.

Bioworld Molecular Weight: ~ 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD100 expression in Jurkat (A) whole cell lysates., Immunofluorescent analysis of CD100 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Alternative Name: C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; GR3; CD100

Immunogen: A synthetic peptide of human Semaphorin 4D

Conjugate: Unconjugated

Modification: Unmodified