Product Description

CD107a/LAMP1 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0245-500, NCP0245-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins. Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis. The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes. LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regμlating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~37kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P11279

Tag: His-tag

Amino Acid Sequence: GCAATGTTCATGGTGAAAAATGGCAATGGTACCGCATGCATTATGGCCAACTTCAGTGCCGCATTCAGTGTTAATTATGATACCAAAAGCGGTCCGAAAAATATGACCTTCGATCTGCCGAGCGATGCAACCGTGGTTCTGAATCGCAGCAGTTGTGGCAAAGAAAATACCAGCGATCCGAGCCTGGTGATTGCATTCGGTCGTGGTCATACCCTGACCCTGAACTTCACCCGTAATGCCACCCGTTATAGTGTGCAGCTGATGAGCTTCGTGTATAATCTGAGTGATACCCATCTGTTCCCGAATGCAAGTAGCAAAGAAATTAAAACCGTTGAAAGTATCACCGATATTCGTGCAGATATTGATAAAAAATACCGCTGCGTGAGTGGTACCCAGGTGCACATGAATAATGTGACCGTTACCCTGCATGATGCCACCATTCAGGCATATCTGAGTAATAGTAGCTTCAGCCGCGGCGAAACCAGATGTGAACAGGATCGCCCGAGTCCGACCACCGCACCGCCTGCACCTCCGTCACCGAGTCCGAGCCCGGTTCCGAAAAGCCCGAGTGTGGATAAATATAATGTTAGCGGTACCAATGGTACCTGCCTGCTGGCAAGTATGGGCCTGCAGCTGAATCTGACCTATGAACGTAAAGATAATACCACCGTGACCCGTCTGCTGAATATTAATCCGAATAAAACCAGCGCCAGCGGTAGCTGTGGTGCACATCTGGTGACCCTGGAACTGCATAGTGAAGGTACCACCGTGCTGCTGTTCCAGTTCGGTATGAATGCAAGTAGTAGCCGCTTCTTCCTGCAGGGTATTCAGCTGAATACCATTCTGCCGGATGCACGCGATCCGGCCTTCAAAGCAGCCAATGGCAGCCTGCGCGCACTGCAGGCAACCGTTGGCAATAGCTATAAATGCAATGCCGAAGAACATGTGCGCGTTACCAAAGCATTCAGCGTTAATATCTTCAAAGTGTGGGTTCAGGCATTCAAAGTTGAAGGCGGTCAGTTCGGTAGTGTTGAAGAATGCCTGCTGGATGAAAATAGCATG

Expression Vector: pet-22b(+)

Research Use Only