Product Description

CD107a/LAMP1 Recombinant Protein

Catalogue Number: NCP0245-500, NCP0245-1000

Sizes: 500ug, 1mg

Swiss-Prot: P11279

Host: E.coli

Tag: His-tag

AA Sequence: GCAATGTTCATGGTGAAAAATGGCAATGGTACCGCATGCATTATGGCCAACTTCAGTGCCGCATTCAGTGTTAATTATGATACCAAAAGCGGTCCGAAAAATATGACCTTCGATCTGCCGAGCGATGCAACCGTGGTTCTGAATCGCAGCAGTTGTGGCAAAGAAAATACCAGCGATCCGAGCCTGGTGATTGCATTCGGTCGTGGTCATACCCTGACCCTGAACTTCACCCGTAATGCCACCCGTTATAGTGTGCAGCTGATGAGCTTCGTGTATAATCTGAGTGATACCCATCTGTTCCCGAATGCAAGTAGCAAAGAAATTAAAACCGTTGAAAGTATCACCGATATTCGTGCAGATATTGATAAAAAATACCGCTGCGTGAGTGGTACCCAGGTGCACATGAATAATGTGACCGTTACCCTGCATGATGCCACCATTCAGGCATATCTGAGTAATAGTAGCTTCAGCCGCGGCGAAACCAGATGTGAACAGGATCGCCCGAGTCCGACCACCGCACCGCCTGCACCTCCGTCACCGAGTCCGAGCCCGGTTCCGAAAAGCCCGAGTGTGGATAAATATAATGTTAGCGGTACCAATGGTACCTGCCTGCTGGCAAGTATGGGCCTGCAGCTGAATCTGACCTATGAACGTAAAGATAATACCACCGTGACCCGTCTGCTGAATATTAATCCGAATAAAACCAGCGCCAGCGGTAGCTGTGGTGCACATCTGGTGACCCTGGAACTGCATAGTGAAGGTACCACCGTGCTGCTGTTCCAGTTCGGTATGAATGCAAGTAGTAGCCGCTTCTTCCTGCAGGGTATTCAGCTGAATACCATTCTGCCGGATGCACGCGATCCGGCCTTCAAAGCAGCCAATGGCAGCCTGCGCGCACTGCAGGCAACCGTTGGCAATAGCTATAAATGCAATGCCGAAGAACATGTGCGCGTTACCAAAGCATTCAGCGTTAATATCTTCAAAGTGTGGGTTCAGGCATTCAAAGTTGAAGGCGGTCAGTTCGGTAGTGTTGAAGAATGCCTGCTGGATGAAAATAGCATG

Restriction Sites: NdeI-XhoI

Background: Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins. Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis. The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes. LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~37kDa

Notes: For research use only, not for use in diagnostic procedure.