Product Description

CD109 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0172P-50, NCP0172P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q6YHK3

Host: Rabbit

Reactivity: Human

Applications: WB, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200

Background: CD109 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein that belongs to the alpha2-macroglobulin family of thioester containing proteins. CD109 is associated with TGF-beta receptor I (TbRI) and inhibits TGF-beta signaling. Cleavage of CD109 at its Furin cleavage site results in the release of its large amino-terminal domain, which then binds to the TGF-beta receptor I to inhibit TGF-beta signaling. CD109 is expressed on a subset of CD34+ bone marrow cells and mesenchymal stem cells, activated platelets, activated T cells, endothelial cells, and a wide variety of tumors. Elevated CD109 expression has been considered a diagnostic/prognostic marker for several types of cancers.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD109 polyclonal antibody detects endogenous levels of CD109 protein.

Bioworld Molecular Weight: ~ 180 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD109 polyclonal antibody at 1:1000 dilution
Lane 1: A549 cell membrane lysate (24ug)
Lane 2: A375 whole cell lysate (24ug), Immunofluorescence analysis of A549 cells using CD109 pAb at dilution of 1:200.

Alternative Name: CD109 antigen; 150 kDa TGF-beta-1-binding protein; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; Platelet-specific Gov antigen; p180; r150; CD109; CPAMD7

Immunogen: Recombinant protein of human CD109.

Conjugate: Unconjugated

Modification: Unmodified