Product Description

CD114/CSF3R Polyclonal Antibody

Catalogue Numbers: NCP0250P-50, NCP0250P-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q99062

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Granulocyte colony-stimulating factor receptor (G-CSF-R) is a transmembrane protein comprised of an immunoglobulin-like (Ig-like) domain, a cytokine receptor-homologous (CRH) domain, and three fibronectin type III (FN III) domains. G-CSF-R is expressed on all granulocytic lineages, including progenitor cells, and has been detected on monocytes, T and B lymphocytes, as well as non-hematopoietic tissues including cardiomyocytes and neural stem cells. The primary ligand for G-CSF-R is the cytokine granulocyte colony-stimulating factor (G-CSF). G-CSF-R has no intrinsic tyrosine kinase activity; ligand binding induces conformational changes in the receptor, leading to activation of the Jak/Stat, PI3K/Akt, and MAPK pathways. G-CSF induces differentiation and proliferation of myeloid progenitor cells into neutrophils. Multiple diseases have been associated with mutations of the G-CSF-R gene, CSF3R, including severe congenital neutropenia (SCN), chronic neutrophilic leukemia (CNL), and atypical chronic myeloid leukemia (aCML).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD114/CSF3R polyclonal antibody detects endogenous levels of CD114/CSF3R protein.

Extra Notes: Western blot (WB) analysis of CD114/CSF3R polyclonal antibody at 1:1000 dilution
Lane1:A549 whole cell lysate(30ug)
Lane2:SP2/0 whole cell lysate(30ug)
Lane3:The Brain tissue lysate of Mouse(30ug)

Bioworld Molecular Weight: ~ 105 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Granulocyte colony-stimulating factor receptor; G-CSF receptor; G-CSF-R; CD_antigen: CD114; CSF3R; GCSFR

Immunogen: Recombinant protein of human CD114/CSF3R.

Conjugate: Unconjugated

Modification: Unmodified