CD114/CSF3R Recombinant Protein - NCP0250

CD114/CSF3R Recombinant Protein

Catalogue Number: NCP0250-500, NCP0250-1000

Sizes: 500ug, 1mg

Swiss-Prot: Q99062

Host: E.coli

Tag: His-tag

AA Sequence: GAATGTGGCCATATTAGTGTGAGCGCCCCGATTGTGCATCTGGGTGATCCGATTACCGCCAGCTGCATTATTAAACAGAATTGCAGCCATCTGGATCCGGAACCGCAGATTCTGTGGCGTCTGGGTGCCGAACTGCAGCCGGGCGGTCGTCAGCAGCGTCTGAGCGATGGCACCCAGGAAAGCATTATTACCCTGCCGCATCTGAATCATACCCAGGCCTTCCTGAGTTGCTGCCTGAATTGGGGTAATAGCCTGCAGATTCTGGATCAGGTGGAACTGCGTGCAGGTTATCCGCCGGCCATTCCGCATAATCTGAGCTGCCTGATGAATCTGACCACCAGTAGCCTGATCTGTCAGTGGGAACCGGGTCCGGAAACACATCTGCCGACCAGCTTCACCCTGAAAAGCTTCAAAAGCCGTGGTAATTGTCAGACCCAGGGTGATAGCATTCTGGATTGTGTTCCGAAAGATGGTCAGAGTCATTGCTGTATTCCGCGTAAACATCTGCTGCTGTATCAGAATATGGGTATCTGGGTTCAGGCCGAAAATGCCCTGGGCACCAGCATGAGCCCGCAGCTGTGCCTGGATCCGATGGATGTTGTGAAACTGGAACCGCCGATGCTGCGCACCATGGATCCGAGCCCGGAAGCAGCACCGCCGCAGGCAGGTTGCCTGCAGCTGTGTTGGGAACCGTGGCAGCCGGGTCTGCATATTAATCAGAAATGCGAACTGCGTCATAAACCGCAGCGCGGTGAAGCCAGCTGGGCCTTAGTGGGTCCGCTGCCGCTGGAAGCCCTGCAGTATGAACTGTGTGGTCTGCTGCCGGCCACCGCCTATACCCTGCAGATTCGCTGCATTCGCTGGCCGCTGCCGGGCCATTGGAGCGATTGGAGCCCGAGCCTGGAACTGCGTACCACCGAACGCGCCCCGACCGTGCGTCTGGATACCTGGTGGCGCCAGCGCCAGCTGGATCCGCGTACCGTGCAGCTGTTCTGGAAACCGGTGCCGCTGGAAGAAGATAGTGGCCGCATTCAGGGCTATGTTGTGAGCTGGCGCCCGAGCGGCCAGGCAGGTGCAATTCTGCCGCTGTGCAATACCACCGAACTGAGTTGCACCTTCCATCTGCCGAGCGAAGCCCAGGAAGTGGCACTGGTGGCATATAATAGCGCCGGTACCAGTCGTCCGACCCCGGTTGTGTTCAGCGAAAGCCGTGGTCCGGCACTGACCCGTCTGCATGCAATGGCCCGTGATCCGCATAGCCTGTGGGTGGGCTGGGAACCGCCGAATCCGTGGCCGCAGGGCTATGTGATTGAATGGGGCCTGGGCCCGCCGAGTGCAAGTAATAGTAATAAAACCTGGCGTATGGAACAGAATGGCCGCGCCACCGGCTTCCTGCTGAAAGAAAATATTCGTCCGTTCCAGCTGTATGAAATTATTGTGACCCCGCTGTATCAGGATACCATGGGTCCGAGCCAGCATGTGTATGCATATAGCCAGGAAATGGCCCCGAGCCATGCACCGGAACTGCATCTGAAACATATTGGTAAAACCTGGGCACAGCTGGAATGGGTTCCGGAACCGCCGGAACTGGGTAAAAGCCCGCTGACCCATTATACCATCTTCTGGACCAATGCCCAGAATCAGAGCTTCAGCGCCATTCTGAATGCAAGCAGCCGCGGCTTCGTGCTGCATGGTCTGGAACCGGCAAGTCTGTATCATATTCATCTGATGGCAGCAAGCCAGGCAGGTGCGACCAATAGTACCGTTCTGACCCTGATGACCCTGACCCCGGAAGGCAGCGAACTGCAT

Restriction Sites: NdeI-XhoI

Background: The diverse biological activities of G-CSF are initiated by the binding of G-CSF to a specific receptor (G-CSFR) that belongs to the cytokine/hematopoietic receptor superfamily. In contrast to the majority of hematopoietic receptors that are activated through the formation of heteromeric complexes composed of α, β and sometimes γ subunits, G-CSFR proteins are believed to form homodimeric complexes upon ligand binding. Four distinct alternative splice variants of G-CSFR have been described, one of which exists as a soluble receptor protein. Although G-CSFR lacks consensus motifs in its cytoplasmic domains that are characteristic of kinase activities, certain sequences have been identified that are conserved among several members of the cytokine receptor superfamily. For example, the carboxy terminal regions of G-CSFR contain a domain, designated box 3, that is only shared with the IL-6R subunit, gp130.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~66kDa

Notes: For research use only, not for use in diagnostic procedure.