Product Description

CD115/CSF1R Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0251-500, NCP0251-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Macrophage-colony stimμlating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2. Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems. The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma and breast cancer.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~55kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P07333

Tag: His-tag

Amino Acid Sequence: ATCCCGGTTATTGAACCGAGTGTTCCGGAACTGGTGGTTAAACCGGGTGCAACCGTTACCCTGCGCTGTGTGGGTAATGGTAGCGTTGAATGGGATGGTCCGCCGAGTCCGCATTGGACCCTGTATAGCGATGGCAGCAGCAGCATTCTGAGCACCAATAATGCCACCTTCCAGAATACCGGTACCTATCGCTGCACCGAACCGGGCGATCCGCTGGGTGGCAGCGCTGCAATTCATCTGTATGTTAAAGATCCGGCACGTCCGTGGAATGTTCTGGCACAGGAAGTTGTTGTGTTCGAAGATCAGGATGCACTGCTGCCGTGCCTGCTGACCGATCCGGTGCTGGAAGCAGGTGTTAGTCTGGTGCGTGTGCGCGGCCGTCCGCTGATGCGTCATACCAATTATAGCTTCAGTCCGTGGCATGGCTTCACCATTCATCGCGCAAAATTCATTCAGAGTCAGGATTATCAGTGTAGTGCACTGATGGGTGGTCGTAAAGTGATGAGCATTAGCATTCGCCTGAAAGTGCAGAAAGTTATTCCGGGTCCGCCGGCCCTGACCCTGGTTCCTGCAGAACTGGTTCGTATTCGTGGCGAAGCCGCCCAGATTGTGTGTAGTGCCAGCAGTGTGGATGTTAACTTCGATGTGTTCCTGCAGCATAATAATACCAAACTGGCCATTCCGCAGCAGAGTGACTTCCATAATAATCGTTATCAGAAAGTGCTGACCCTGAATCTGGATCAGGTGGACTTCCAGCATGCCGGCAATTATAGCTGTGTTGCCAGCAATGTGCAGGGCAAACATAGCACCAGCATGTTCTTCCGTGTTGTGGAAAGCGCCTATCTGAATCTGAGTAGTGAACAGAATCTGATTCAGGAAGTGACCGTGGGCGAAGGCCTGAATCTGAAAGTTATGGTGGAAGCATATCCGGGCCTGCAGGGCTTCAATTGGACCTATCTGGGCCCGTTCAGTGATCATCAGCCGGAACCGAAACTGGCAAATGCAACCACCAAAGATACCTATCGCCATACCTTCACCCTGAGTCTGCCGCGTCTGAAACCGAGTGAAGCCGGCCGTTATAGCTTCCTGGCCCGTAATCCGGGTGGCTGGCGCGCACTGACCTTCGAACTGACCCTGCGTTATCCGCCGGAAGTGAGTGTTATCTGGACCTTCATTAATGGCAGTGGCACCCTGCTGTGTGCCGCCAGTGGTTATCCGCAGCCGAATGTGACCTGGCTGCAGTGCAGTGGCCATACCGATCGCTGTGATGAAGCCCAGGTTCTGCAGGTGTGGGATGATCCGTATCCGGAAGTTCTGAGCCAGGAACCGTTCCATAAAGTGACCGTGCAGAGCCTGCTGACCGTGGAAACCTTAGAACATAATCAGACCTATGAATGCCGCGCACATAATAGTGTTGGTAGCGGTAGCTGGGCCTTCATTCCGATTAGTGCAGGCGCCCATACCCATCCGCCGGATGAATTCCTGTTCACCCCG

Expression Vector: pet-22b(+)

Research Use Only