CD117/KIT Recombinant Protein - NCP0253

CD117/KIT Recombinant Protein

Catalogue Number: NCP0253-500, NCP0253-1000

Sizes: 500ug, 1mg

Swiss-Prot: P10721

Host: E.coli

Tag: His-tag

AA Sequence: CAGCCTAGTGTTAGCCCGGGTGAACCGAGCCCGCCGAGCATTCATCCGGGCAAAAGCGATCTGATTGTTCGTGTTGGTGATGAAATTCGTCTGCTGTGCACCGATCCGGGCTTCGTGAAATGGACCTTCGAAATTCTGGATGAAACCAATGAAAATAAGCAGAATGAATGGATTACCGAAAAAGCAGAAGCCACCAATACCGGCAAATATACCTGCACCAATAAACATGGTCTGAGTAATAGTATCTATGTGTTCGTTCGCGATCCGGCCAAACTGTTCCTGGTGGATCGTAGCCTGTATGGTAAAGAAGATAATGATACCCTGGTGCGCTGTCCGCTGACCGATCCGGAAGTTACCAATTATAGTCTGAAAGGTTGCCAGGGCAAACCGCTGCCGAAAGATCTGCGCTTCATTCCGGATCCGAAAGCAGGCATTATGATTAAAAGCGTTAAACGCGCATATCATCGTCTGTGTCTGCATTGCAGCGTGGATCAGGAAGGTAAAAGTGTTCTGAGCGAAAAATTCATTCTGAAAGTGCGCCCGGCATTCAAAGCAGTTCCGGTTGTGAGCGTGAGTAAAGCAAGTTATCTGCTGCGCGAAGGCGAAGAATTCACCGTGACCTGTACCATTAAAGATGTGAGTAGCAGCGTGTATAGTACCTGGAAACGTGAAAATAGTCAGACCAAACTGCAGGAAAAATATAATAGTTGGCATCATGGTGACTTCAATTATGAACGTCAGGCCACCCTGACCATTAGCAGCGCCCGCGTTAATGATAGCGGCGTGTTCATGTGTTATGCAAATAATACCTTCGGTAGCGCCAATGTGACCACCACCCTGGAAGTTGTGGATAAAGGCTTCATTAATATCTTCCCGATGATTAATACCACCGTGTTCGTGAATGATGGTGAAAATGTGGATCTGATTGTGGAATATGAAGCATTCCCGAAACCGGAACATCAGCAGTGGATCTATATGAATCGCACCTTCACCGATAAATGGGAAGATTATCCGAAAAGCGAAAATGAAAGCAATATTCGTTACGTGAGTGAACTGCATCTGACCCGCCTGAAAGGCACCGAAGGCGGCACCTATACCTTCCTGGTGAGTAATAGCGATGTGAATGCAGCAATTGCCTTCAATGTGTATGTGAATACCAAACCGGAAATTCTGACCTATGATCGCCTGGTGAATGGCATGCTGCAGTGCGTTGCAGCCGGCTTCCCGGAACCGACCATTGATTGGTACTTCTGCCCGGGTACCGAACAGCGTTGTAGCGCCAGTGTGCTGCCGGTGGATGTTCAGACCCTGAATAGCAGCGGCCCGCCGTTCGGTAAACTGGTTGTTCAGAGCAGTATTGATAGTAGTGCCTTCAAACATAATGGCACCGTGGAATGCAAAGCCTATAATGATGTGGGTAAAACCAGCGCATACTTCAACTTCGCCTTCAAAGGTAATAATAAAGAACAGATTCACCCGCATACCCTGTTCACCCCG

Restriction Sites: NdeI-XhoI

Background: c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors. It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells. Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors. Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~55kDa

Notes: For research use only, not for use in diagnostic procedure.