Product Description

CD117 Monoclonal Antibody

Catalogue Numbers: MB65747-50, MB65747-100

Sizes: 50µl, 100µl

Product: Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P10721

Host: Mouse

Reactivity: Human

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors. It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells. Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors. Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo.

Purification and Purity: The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes human CD117

Extra Notes: Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD117 Antibody, followed by anti-mouse IgG PE.

Bioworld Molecular Weight: ~ 109 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: SCFR; Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD antigen CD117

Immunogen: Native purified human CD117.

Conjugate: Unconjugated

Modification: Unmodified