Product Description

CD118 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0254-500, NCP0254-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: IL-6 activates intracellμlar signaling through binding a receptor consisting of an ligand-binding protein (IL-6R) and a second protein. IL-6 first binds to IL-6R which subsequently associates with a gp130 dimer. The active signaling complex consists of at minimum IL-6, IL-6R and a dimer of two gp130 proteins that are linked by a disμlfide bond. A soluble form of IL-6R is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecμle that can actively participate in cell-to-cell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor (LIFR) with low affinity and to a complex of the LIF receptor
and gp130 with high affinity while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~87kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P42702

Tag: His-tag

Amino Acid Sequence: CAGAAAAAGGGCGCCCCGCATGATCTGAAATGCGTGACCAATAATCTGCAGGTGTGGAATTGCAGCTGGAAAGCCCCGAGTGGTACCGGCCGTGGCACCGATTATGAAGTGTGCATTGAAAATCGTAGCCGCAGTTGTTATCAGCTGGAAAAAACCAGCATTAAAATTCCGGCCCTGAGCCATGGTGATTATGAAATTACCATTAATAGCCTGCATGACTTCGGTAGTAGCACCAGTAAATTCACCCTGAATGAACAGAATGTGAGCCTGATTCCGGATACCCCGGAAATTCTGAATCTGAGCGCCGACTTCAGCACCAGTACCCTGTATCTGAAATGGAATGATCGTGGTAGTGTGTTCCCGCATCGTAGTAATGTTATCTGGGAAATTAAAGTTCTGCGCAAAGAAAGTATGGAACTGGTGAAACTGGTGACCCATAATACCACCCTGAATGGTAAAGATACCCTGCATCATTGGAGTTGGGCAAGCGATATGCCGCTGGAATGCGCAATTCACTTCGTGGAAATTCGTTGCTATATTGATAATCTGCACTTCAGTGGCCTGGAAGAATGGAGTGATTGGAGTCCGGTTAAAAATATTAGTTGGATTCCGGATAGTCAGACCAAAGTGTTCCCGCAGGATAAAGTGATTCTGGTTGGCAGCGATATTACCTTCTGTTGCGTTAGCCAGGAAAAAGTGCTGAGTGCCCTGATTGGTCATACCAATTGTCCGCTGATTCATCTGGATGGCGAAAATGTGGCAATTAAAATTCGCAATATCAGCGTTAGCGCAAGTAGTGGCACCAATGTGGTGTTCACCACCGAAGATAATATCTTCGGTACCGTTATCTTCGCCGGTTATCCGCCGGATACCCCTCAGCAGCTGAATTGCGAAACACATGATCTGAAGGAAATTATCTGTAGCTGGAATCCGGGCCGTGTGACCGCCCTGGTGGGTCCTCGTGCAACCAGCTATACCCTGGTGGAAAGCTTCAGCGGCAAATATGTGCGCCTGAAACGTGCCGAAGCACCGACCAATGAAAGCTATCAGCTGCTGTTCCAGATGCTGCCGAATCAGGAAATCTATAACTTCACCCTGAACGCCCATAATCCGCTGGGCCGCAGTCAGAGTACCATTCTGGTTAATATTACCGAAAAAGTGTATCCGCATACCCCGACCAGCTTCAAAGTTAAAGATATTAATAGCACCGCCGTTAAACTGAGTTGGCATCTGCCGGGTAACTTCGCAAAAATTAACTTCCTGTGTGAAATTGAAATCAAGAAAAGCAATAGCGTTCAGGAACAGCGCAATGTTACCATTAAAGGCGTTGAAAATAGTAGCTATCTGGTTGCCCTGGATAAACTGAATCCGTATACCCTGTATACCTTCCGTATTCGCTGCAGTACCGAAACCTTCTGGAAATGGAGTAAATGGAGCAATAAAAAACAGCATCTGACCACCGAAGCCAGCCCGAGTAAAGGTCCGGATACCTGGCGTGAATGGAGCAGCGATGGCAAAAATCTGATTATCTATTGGAAACCGCTGCCGATTAATGAAGCAAATGGTAAAATTCTGAGCTATAATGTGAGCTGTAGCAGCGATGAAGAAACACAAAGTCTGAGCGAAATTCCGGATCCGCAGCATAAAGCAGAAATTCGCCTGGATAAAAATGATTATATCATCAGCGTTGTGGCCAAAAATAGTGTGGGTAGCAGCCCGCCGAGCAAAATTGCAAGCATGGAAATTCCGAATGATGATCTGAAAATTGAACAGGTTGTTGGTATGGGTAAAGGTATTCTGCTGACCTGGCATTATGATCCGAATATGACCTGCGATTATGTGATTAAATGGTGCAATAGCAGTCGTAGTGAACCGTGCCTGATGGATTGGCGTAAAGTTCCGAGTAATAGCACCGAAACCGTGATTGAAAGCGATGAATTCCGTCCGGGTATTCGCTATAACTTCTTCCTGTATGGTTGTCGCAATCAGGGTTATCAGCTGTTACGCAGTATGATTGGTTATATTGAAGAACTGGCCCCGATTGTGGCACCGAACTTCACCGTGGAAGATACCAGCGCAGATAGCATTCTGGTTAAATGGGAAGATATTCCGGTTGAAGAACTGCGTGGCTTCCTGCGCGGTTATCTGTTCTACTTCGGTAAAGGTGAACGTGATACCAGTAAAATGCGCGTTCTGGAAAGTGGTCGTAGCGATATTAAAGTTAAAAATATCACCGACATCAGCCAGAAAACCTTACGTATTGCCGATCTGCAGGGCAAAACCAGCTATCATCTGGTTCTGCGCGCATATACCGATGGTGGTGTTGGTCCGGAAAAAAGCATGTATGTTGTTACCAAAGAAAACAGC

Expression Vector: pet-22b(+)

Research Use Only