Product Description

CD119 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0255P-50, NCP0255P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P15260

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200

Background: Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation Associates with transmembrane accessory factor IFNGR2 to form a functional receptor.Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription.STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (By similarity).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD119 polyclonal antibody detects endogenous levels of CD119 protein.

Bioworld Molecular Weight: ~ 45-90 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD119 polyclonal antibody at 1:1000 dilution
Lane 1: The Liver tissue lysate of Rat (30ug)
Lane 2: SP2/0 cell membrane lysate (30ug)
Lane 3: HepG2 whole cell lysate (30ug)
Lane 4: MCF-7 cell membrane lysate (30ug), Immunofluorescence analysis of MCF-7 cells using CD119 pAb at dilution of 1:200.

Alternative Name: Interferon gamma receptor 1; IFN-gamma receptor; IFN-gamma-R1; CDw119; Interferon gamma receptor alpha-chain; IFN-gamma-R-alpha; CD119

Immunogen: Recombinant protein of human CD119.

Conjugate: Unconjugated

Modification: Unmodified