Product Description

CD11b Rat Monoclonal Antibody

Catalogue Numbers:MB65829-50, MB65829-100

Product: Rat IgG2b kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P11215

Host: Rat

Reactivity: Human, Mouse

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits. CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia. CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the blood stream to tissues. Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematous (SLE). The M1/70 antibody is widely used as a marker for CD11b expression on neutrophils, macrophages, NK cells, and dendritic cells (DC).

Purification and Purity: The monoclonal antibody was affinity-purified from rat antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bioworld Molecular Weight: ~ 127 kDa

Specificity: Recognizes mouse CD11b

Note: For research use only, not for use in diagnostic procedure.
Alternative Name: CD11B; CR3A; Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD11b

Immunogen: C57BL/10 splenocytes

Conjugate: Unconjugated

Modification: Unmodified