Product Description

CD126 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0257-500, NCP0257-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The IL-6 receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130. Binding of IL-6 to IL-6Rα resμlts in dimerization of receptor with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17. In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA. Soluble IL-6Rα binds to IL-6 and can stimμlate signaling via membrane bound gp130 in a process known as “trans-signaling”. It is through trans-signaling that IL-6 stimμlates cells that do not express membrane bound IL-6Rα.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~44kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P08887

Tag: His-tag

Amino Acid Sequence: CTGGCTCCTCGTCGCTGTCCGGCCCAGGAAGTTGCCCGTGGTGTGCTGACCAGCCTGCCGGGTGATAGCGTTACCCTGACCTGCCCGGGCGTTGAACCGGAAGATAATGCAACCGTTCATTGGGTGCTGCGTAAACCGGCCGCAGGCAGCCATCCGAGCCGTTGGGCTGGTATGGGTCGCCGTCTGCTGCTGCGCAGCGTGCAGCTGCATGATAGCGGCAATTATAGTTGCTATCGTGCCGGTCGCCCGGCAGGCACCGTTCATCTGCTGGTGGATGTGCCGCCGGAAGAACCGCAGCTGAGCTGCTTCCGCAAAAGCCCGCTGAGTAATGTGGTGTGCGAATGGGGCCCGCGTAGTACCCCGAGTCTGACCACCAAAGCCGTTCTGCTGGTGCGCAAATTCCAGAATAGCCCGGCCGAAGACTTCCAGGAACCGTGCCAGTATAGTCAGGAAAGTCAGAAATTCAGTTGCCAGCTGGCAGTGCCGGAAGGCGATAGCAGCTTCTATATTGTGAGTATGTGTGTTGCCAGCAGCGTTGGTAGCAAATTCAGTAAAACACAAACCTTCCAGGGTTGTGGCATTCTGCAGCCGGATCCGCCGGCCAATATTACCGTTACCGCAGTTGCCCGCAATCCGCGCTGGCTGAGTGTGACCTGGCAGGATCCGCATAGCTGGAATAGCAGCTTCTACCGCCTGCGCTTCGAACTGCGTTATCGCGCAGAACGCAGTAAAACCTTCACCACCTGGATGGTTAAAGATCTGCAGCATCATTGCGTGATTCATGATGCATGGAGTGGCCTGCGTCATGTGGTTCAGCTGCGCGCACAGGAAGAATTCGGCCAGGGTGAATGGAGTGAATGGAGCCCGGAAGCAATGGGCACCCCGTGGACCGAAAGCCGCAGCCCTCCGGCTGAAAATGAAGTGAGCACCCCGATGCAGGCACTGACCACCAATAAAGATGATGATAATATTCTGTTCCGCGATAGCGCCAATGCCACCAGTCTGCCGGTTCAGGATAGTAGCAGCGTGCCGCTGCCG

Expression Vector: pet-22b(+)

Research Use Only