CD13 Polyclonal Antibody-BS90213

CD13 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90213-50, BS90213-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P15144(Human) P97449(Mouse) P15684(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IHC, IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: CD13, or aminopeptidase N, is a type II transmembrane glycoprotein that is expressed on most cells of Myeloid origin, including monocytes, basophils, eosinophils, neutrophils and Myeloid leukemias. CD13 is also found on certain epithelial cells, fibroblasts and osteoclasts. CD13 acts as a zinc-binding metalloprotease that plays a role in digestion and may function in the inactivation of some regulatory peptides such as enkephalins. CD13 may play a role in the invasion of cancer cells by enhancing their invasive capacity and metastatic behavior. The activity of CD13 can be inactivated using specific inhibitors that evoke apoptosis of CD13-positive cancer cells. Basic fibroblast growth factor (bFGF) expression upregulates CD13 expression in human melanoma cells by activating both the Myeloid and the epithelial CD13 promoter.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD13 polyclonal antibody detects endogenous levels of CD13 protein.

Bioworld Molecular Weight: 150 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: THP-1
Lane 2: human heart
Lane 3: U937, ICC staining CD13 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: AP-N, hAPN, AP-M, ANPEP, Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13, CD13 antigen, gp150, hAPN, LAP 1, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified