Product Description

CD133 Monoclonal Antibody

Catalogue Numbers: MB66678-50, MB66678-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: O43490

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD133 protein.

Extra Notes: Western blot analysis of CD133 expression in CaCo2 (A) whole cell lysates.

Bioworld Molecular Weight: ~ 133 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: PROML1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133

Immunogen: Purified recombinant human Prominin-1 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified