Product Description

CD133 Polyclonal Antibody

Catalogue Numbers: NCP0261P-50, NCP0261P-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: O43490

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD133 polyclonal antibody detects endogenous levels of CD133 protein.

Extra Notes: Western blot (WB) analysis of CD133 polyclonal antibody at 1:1000 dilution
Lane1:L02 whole cell lysate(30ug)
Lane2:HepG2 whole cell lysate(30ug)
Lane3:Panc1 whole cell lysate(30ug)
Lane4:H1792 whole cell lysate(30ug)

Bioworld Molecular Weight: ~ 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Prominin-1; Antigen AC133; Prominin-like protein 1; CD133; PROM1; PROML1; MSTP061

Immunogen: Recombinant protein of human CD133.

Conjugate: Unconjugated

Modification: Unmodified