Product Description

CD133 Recombinant Protein

Catalogue Number: NCP0261-500, NCP0261-1000

Sizes: 500ug, 1mg

Swiss-Prot: O43490

Host: E.coli

Tag: His-tag

AA Sequence: GGTGCAAATGTGGAAAAACTGATCTGTGAACCGTATACCAGTAAAGAACTGTTCCGCGTTCTGGATACCCCGTATCTGCTGAATGAAGATTGGGAATATTATCTGAGTGGTAAACTGTTCAATAAGAGTAAAATGAAGCTGACCTTCGAACAGGTGTATAGCGATTGTAAAAAAAATCGCGGTACCTATGGCACCCTGCATCTGCAGAATAGCTTCAATATTAGTGAACATCTGAACATTAACGAGCATACCGGCAGTATTAGCAGCGAACTGGAAAGTCTGAAAGTGAATCTGAATATCTTCCTGCTGGGCGCAGCCGGTCGTAAAAATCTGCAGGACTTCGCCGCCTGCGGCATTGATCGTATGAATTATGATAGTTATCTGGCCCAGACCGGCAAAAGTCCGGCCGGCGTTAATCTGCTGAGCTTCGCATACGATCTGGAAGCAAAAGCCAATAGCCTGCCGCCGGGCAATCTGCGCAATAGCCTGAAACGCGATGCCCAGACCATTAAAACCATTCATCAGCAGCGCGTGCTGCCGATTGAACAGAGCCTGAGCACCCTGTATCAGAGCGTTAAAATTCTGCAGCGTACCGGCAATGGCCTGCTGGAACGCGTTACCCGTATTCTGGCCAGCCTGGACTTCGCCCAGAACTTCATTACCAATAATACCAGTAGTGTTATCATCGAAGAAACCAAAAAATACGGCCGTACCATTATTGGCTACTTCGAACATTATCTGCAGTGGATTGAATTCAGTATTAGTGAAAAAGTGGCAAGCTGCAAACCGGTGGCCACCGCCCTGGATACCGCAGTGGATGTGTTCCTGTGTAGCTATATTATTGATCCGCTGAAT

Restriction Sites: NdeI-XhoI

Background: CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicated molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~31kDa

Notes: For research use only, not for use in diagnostic procedure.