CD135 Recombinant Protein - NCP0262

CD135 Recombinant Protein

Catalogue Number: NCP0262-500, NCP0262-1000

Sizes: 500ug, 1mg

Swiss-Prot: P36888

Host: E.coli

Tag: His-tag

AA Sequence: AACCAGGATCTGCCGGTTATTAAATGTGTGCTGATTAATCATAAGAACAACGATAGCAGCGTGGGCAAAAGCAGCAGCTATCCGATGGTTAGCGAAAGTCCGGAAGATCTGGGTTGTGCACTGCGTCCGCAGAGTAGTGGCACCGTGTATGAAGCCGCCGCAGTGGAAGTTGATGTGAGTGCAAGTATTACCCTGCAGGTGCTGGTTGATGCCCCGGGCAATATTAGTTGTCTGTGGGTGTTCAAACATAGTAGCCTGAATTGTCAGCCGCACTTCGATCTGCAGAATCGCGGTGTGGTTAGTATGGTTATTCTGAAAATGACCGAAACACAAGCCGGCGAATATCTGCTGTTCATTCAGAGCGAAGCAACCAATTATACCATTCTGTTCACCGTGAGCATTCGTAATACCCTGCTGTATACCCTGCGTCGTCCGTACTTCCGCAAAATGGAAAATCAGGATGCCCTGGTGTGTATTAGCGAAAGCGTTCCGGAACCGATTGTGGAATGGGTGCTGTGCGATAGCCAGGGTGAAAGCTGTAAAGAAGAAAGTCCGGCAGTGGTGAAAAAAGAAGAAAAAGTTCTGCATGAACTGTTCGGCACCGATATTCGCTGCTGCGCACGTAATGAACTGGGTCGTGAATGTACCCGTCTGTTCACCATTGATCTGAATCAGACCCCGCAGACCACCCTGCCGCAGCTGTTCCTGAAAGTTGGCGAACCGCTGTGGATTCGTTGTAAAGCCGTGCATGTGAATCATGGCTTCGGTCTGACCTGGGAACTGGAAAATAAAGCACTGGAAGAAGGTAATTACTTCGAAATGAGTACCTATAGTACCAATCGTACCATGATTCGTATTCTGTTCGCATTCGTTAGCAGTGTTGCACGCAATGATACCGGCTATTATACCTGCAGCAGCAGTAAACATCCGAGCCAGAGCGCCCTGGTTACCATTGTTGAAAAAGGCTTCATTAATGCCACCAATAGTAGCGAAGATTATGAAATTGATCAGTATGAAGAGTTCTGCTTCAGTGTTCGCTTCAAAGCATATCCGCAGATTCGTTGTACCTGGACCTTCAGTCGCAAAAGCTTCCCGTGCGAACAGAAAGGCCTGGATAATGGTTATAGTATTAGCAAATTCTGCAACCATAAGCATCAGCCGGGTGAATATATCTTCCATGCAGAAAATGATGATGCCCAGTTCACCAAAATGTTCACCCTGAATATTCGTCGCAAACCGCAGGTTCTGGCAGAAGCCAGCGCCAGTCAGGCCAGCTGCTTCAGCGATGGTTATCCGCTGCCGAGTTGGACCTGGAAAAAATGCAGCGATAAAAGCCCGAATTGCACCGAAGAAATTACCGAAGGCGTGTGGAATCGCAAAGCCAATCGTAAAGTGTTCGGCCAGTGGGTTAGCAGTAGTACCCTGAATATGAGTGAAGCAATTAAAGGCTTCCTGGTTAAATGTTGCGCATATAATAGCCTGGGCACCAGCTGTGAAACCATTCTGCTGAATAGCCCGGGCCCGTTCCCGTTCATTCAGGATAATATTAGC

Restriction Sites: NdeI-XhoI

Background: FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system. FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets. The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation. Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~57kDa

Notes: For research use only, not for use in diagnostic procedure.