Product Description

CD135 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0262-500, NCP0262-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system. FLT3 is activated after binding with its ligand FL, which resμlts in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets. The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimμlation. Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regμlation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~57kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P36888

Tag: His-tag

Amino Acid Sequence: AACCAGGATCTGCCGGTTATTAAATGTGTGCTGATTAATCATAAGAACAACGATAGCAGCGTGGGCAAAAGCAGCAGCTATCCGATGGTTAGCGAAAGTCCGGAAGATCTGGGTTGTGCACTGCGTCCGCAGAGTAGTGGCACCGTGTATGAAGCCGCCGCAGTGGAAGTTGATGTGAGTGCAAGTATTACCCTGCAGGTGCTGGTTGATGCCCCGGGCAATATTAGTTGTCTGTGGGTGTTCAAACATAGTAGCCTGAATTGTCAGCCGCACTTCGATCTGCAGAATCGCGGTGTGGTTAGTATGGTTATTCTGAAAATGACCGAAACACAAGCCGGCGAATATCTGCTGTTCATTCAGAGCGAAGCAACCAATTATACCATTCTGTTCACCGTGAGCATTCGTAATACCCTGCTGTATACCCTGCGTCGTCCGTACTTCCGCAAAATGGAAAATCAGGATGCCCTGGTGTGTATTAGCGAAAGCGTTCCGGAACCGATTGTGGAATGGGTGCTGTGCGATAGCCAGGGTGAAAGCTGTAAAGAAGAAAGTCCGGCAGTGGTGAAAAAAGAAGAAAAAGTTCTGCATGAACTGTTCGGCACCGATATTCGCTGCTGCGCACGTAATGAACTGGGTCGTGAATGTACCCGTCTGTTCACCATTGATCTGAATCAGACCCCGCAGACCACCCTGCCGCAGCTGTTCCTGAAAGTTGGCGAACCGCTGTGGATTCGTTGTAAAGCCGTGCATGTGAATCATGGCTTCGGTCTGACCTGGGAACTGGAAAATAAAGCACTGGAAGAAGGTAATTACTTCGAAATGAGTACCTATAGTACCAATCGTACCATGATTCGTATTCTGTTCGCATTCGTTAGCAGTGTTGCACGCAATGATACCGGCTATTATACCTGCAGCAGCAGTAAACATCCGAGCCAGAGCGCCCTGGTTACCATTGTTGAAAAAGGCTTCATTAATGCCACCAATAGTAGCGAAGATTATGAAATTGATCAGTATGAAGAGTTCTGCTTCAGTGTTCGCTTCAAAGCATATCCGCAGATTCGTTGTACCTGGACCTTCAGTCGCAAAAGCTTCCCGTGCGAACAGAAAGGCCTGGATAATGGTTATAGTATTAGCAAATTCTGCAACCATAAGCATCAGCCGGGTGAATATATCTTCCATGCAGAAAATGATGATGCCCAGTTCACCAAAATGTTCACCCTGAATATTCGTCGCAAACCGCAGGTTCTGGCAGAAGCCAGCGCCAGTCAGGCCAGCTGCTTCAGCGATGGTTATCCGCTGCCGAGTTGGACCTGGAAAAAATGCAGCGATAAAAGCCCGAATTGCACCGAAGAAATTACCGAAGGCGTGTGGAATCGCAAAGCCAATCGTAAAGTGTTCGGCCAGTGGGTTAGCAGTAGTACCCTGAATATGAGTGAAGCAATTAAAGGCTTCCTGGTTAAATGTTGCGCATATAATAGCCTGGGCACCAGCTGTGAAACCATTCTGCTGAATAGCCCGGGCCCGTTCCCGTTCATTCAGGATAATATTAGC

Expression Vector: pet-22b(+)

Research Use Only