CD14 Monoclonal Antibody-MB65751

CD14 Monoclonal Antibody

Catalogue Numbers: MB65751-50, MB65751-100

Sizes: 50µl, 100µl

Product: Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P08571

Host: Mouse

Reactivity: Human

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage. Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein. LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection. The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage .

Purification and Purity: The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes human CD14

Extra Notes: Flow cytometric analysis of human peripheral blood monocytes using Anti-CD14 Antibody, followed by anti-mouse IgG PE.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14)

Immunogen: Native purified human CD14.

Conjugate: Unconjugated

Modification: Unmodified