Product Description

CD14 Polyclonal Antibody

Catalogue Numbers: NCP0176P-50, NCP0176P-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P08571

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:5000~1:10000

Background: Coreceptor for bacterial lipopolysaccharide.In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS).Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response .
Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway .Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- .

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD14 polyclonal antibody detects endogenous levels of CD14 protein.

Extra Notes: Western blot (WB) analysis of CD14 polyclonal antibody at 1:5000 dilution
Lane1:MCF-7 cell membrane lysate(25ug)
Lane2:A549 cell membrane lysate(25ug)

Bioworld Molecular Weight: ~45 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form

Immunogen: Recombinant protein of human CD14.

Conjugate: Unconjugated

Modification: Unmodified