Product Description

CD14 Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0176-500, NCP0176-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage. Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein. LPS induces an upregμlation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection. The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~40kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P08571

Tag: His-tag

Amino Acid Sequence: ACCACCCCTGAACCGTGCGAACTGGATGATGAAGACTTCCGCTGCGTGTGTAACTTCAGTGAACCGCAGCCGGATTGGAGCGAAGCCTTCCAGTGCGTTAGTGCAGTTGAAGTGGAAATTCATGCAGGCGGCCTGAATCTGGAACCGTTCCTGAAACGCGTTGATGCAGATGCAGATCCGCGCCAGTATGCCGATACCGTTAAAGCCCTGCGTGTGCGTCGTCTGACCGTGGGTGCAGCACAGGTGCCGGCACAGCTGCTGGTTGGTGCACTGCGTGTTCTGGCCTATAGTCGCCTGAAAGAACTGACCCTGGAAGATCTGAAAATTACCGGTACCATGCCGCCGCTGCCGCTGGAAGCCACCGGTCTTGCACTGAGCAGCCTGCGTCTGCGTAATGTGAGCTGGGCCACCGGTCGCAGCTGGCTGGCAGAACTGCAGCAGTGGCTGAAACCGGGTCTGAAAGTTCTGAGCATTGCCCAGGCACATAGCCCGGCATTCAGTTGCGAACAGGTTCGCGCCTTCCCGGCACTGACCAGTCTGGATCTGAGTGATAATCCGGGTCTGGGCGAACGCGGTCTGATGGCCGCACTGTGTCCGCATAAATTCCCGGCAATTCAGAATCTGGCACTGCGTAATACCGGTATGGAAACACCTACCGGTGTGTGTGCAGCCCTGGCCGCCGCTGGTGTGCAGCCTCATAGCCTGGATCTGTCACATAATAGCCTGCGCGCCACCGTTAATCCGAGCGCCCCGCGTTGCATGTGGAGCAGCGCACTGAATAGCCTGAATCTGAGCTTCGCAGGTCTGGAACAGGTTCCGAAAGGCCTGCCGGCAAAACTGCGTGTTCTTGATCTGAGCTGTAATCGCCTGAATCGCGCACCGCAGCCGGACGAACTGCCGGAAGTGGATAATCTGACCCTGGATGGTAATCCGTTCCTGGTGCCGGGCACCGCACTGCCTCATGAAGGCAGCATGAAT

Expression Vector: pet-22b(+)

Research Use Only