Product Description

CD142 (Phospho-S290) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64521-50, BS64521-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P13726

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Coagulation Factor V (Factor V, FV, proaccelerin, labile factor) is a 2196 amino acid, single chain glycoprotein that is cleaved by Thrombin to yield an active, Ca2+-dependent dimer that is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates into a Prothrombinase complex, which mediates proteolysis of Prothrombin into active Thrombin. Tissue factor (TF), also designated coagulation Factor III is a cell surface glycoprotein that enables cells to initiate blood coagulation cascades. It functions as a high-affinity receptor for coagulation Factor VII.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD142 (Phospho-S290) polyclonal antibody detects endogenous levels of CD142 protein only when phosphorylated at Ser290.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD142 (Phospho-S290) polyclonal antibody at 1:500 dilution
Lane A: The Brain tissue lysate of Mouse
Lane B: The Lung tissue lysate of Mouse
Lane C: The Liver tissue lysate of Mouse
Lane D: The Liver tissue lysate of Rat

Alternative Name: Tissue factor; TF; Coagulation factor III; Thromboplastin; CD142; F3

Immunogen: Synthetic phosphopeptide derived from human CD142 around the phosphorylation site of Serine 290.

Conjugate: Unconjugated

Modification: Phosphorylation