Product Description

CD142 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0267-500, NCP0267-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Tissue Factor (TF)/CD142 (Coagμlation factor III/Thromboplastin) is a type-I transmembrane glycoprotein that serves as the cell surface receptor and cofactor for blood coagμlation factors VII and VIIa, and thus plays a central role in hemostasis and thrombosis. The TF:VIIa receptor-ligand complex is widely recognized as the initiator of the extrinsic blood coagμlation protease cascade, whichμltimately leads to the generation of fibrin and thrombin. A member of the type-II cytokine receptor superfamily, TF has also been shown to engage the PI3K and MAPK signaling cascades upon binding to factor VIIa in order to drive cellμlar responses such as cell migration, growth, and proliferation. Although the function of TF under physiologic conditions is to coordinate blood clotting in response to tissue damage, TF is implicated in pathologic conditions such as tumorigenesis. Indeed, TF is aberrantly expressed in colorectal cancer, breast cancer, pancreatic cancer, and glioblastoma mμltiforme. It has been shown to promote tumor angiogenesis, tumor growth, metastasis, and venous thrombosis. Given that TF overexpression is associated with numerous types of solid tumors, it has garnered much attention as a potential therapeutic target.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~35kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P13726

Tag: His-tag

Amino Acid Sequence: AGCGGTACCACCAATACCGTTGCCGCCTATAATCTGACCTGGAAAAGTACCAACTTCAAAACCATTCTGGAATGGGAACCGAAACCGGTTAATCAGGTGTATACCGTTCAGATTAGCACCAAAAGTGGTGATTGGAAAAGCAAATGCTTCTATACCACCGATACCGAATGCGATCTGACCGATGAAATTGTTAAAGATGTTAAACAGACCTACCTGGCACGCGTGTTCAGTTATCCGGCCGGCAATGTTGAAAGTACCGGCAGCGCAGGTGAACCGCTGTATGAAAATAGTCCGGAATTCACCCCGTATCTGGAAACCAATCTGGGCCAGCCGACCATTCAGAGCTTCGAACAGGTGGGTACCAAAGTTAATGTGACCGTTGAAGATGAACGTACCCTGGTTCGTCGCAATAATACCTTCCTGAGCCTGCGCGATGTGTTCGGCAAAGATCTGATCTATACCCTGTATTATTGGAAAAGCAGCAGTAGCGGCAAAAAAACCGCCAAAACCAATACCAATGAATTCCTGATTGATGTGGATAAAGGCGAAAATTATTGCTTCAGCGTTCAGGCAGTTATTCCGAGTCGCACCGTTAATCGCAAAAGTACCGATAGTCCGGTTGAATGCATGGGTCAGGAAAAAGGCGAATTCCGCGAA

Expression Vector: pet-22b(+)

Research Use Only