Product Description

CD143 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0268-500, NCP0268-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Angiotensin-converting enzyme (ACE) is a carboxy-terminal dipeptidyl exopeptidase that converts Angiotensin I to the potent vasopressive hormone, Angiotensin II. There are two isoforms of ACE, the pμlmonary ACEP and the testicμlar ACET. ACEP is a glycoprotein expressed in vascμlar endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adμlt testis by developing sperm cells, specifically, late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in fluid/electrolyte homeostasis. Although it bears significant sequence homology to ACE, ACE2
shows a more restricted pattern of expression. ACE is expressed ubiquitously throughout the vascμlature while ACE2 is expressed only in cardiac, renal and testicμlar cells.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~66kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P12821

Tag: His-tag

Amino Acid Sequence: CTGGATCCGGGTCTGCAGCCGGGTAACTTCAGTGCAGATGAAGCAGGTGCACAGCTGTTCGCACAGAGCTATAATAGTAGCGCAGAACAGGTTCTGTTCCAGAGCGTTGCCGCAAGTTGGGCCCATGATACCAATATTACCGCCGAAAATGCCCGTCGCCAGGAAGAAGCCGCCCTGCTGAGTCAGGAATTCGCAGAAGCCTGGGGCCAGAAAGCAAAAGAACTGTATGAACCGATCTGGCAGAACTTCACCGATCCGCAGCTGCGTCGCATTATTGGTGCAGTGCGTACCCTGGGTAGCGCAAATCTGCCGCTGGCAAAACGTCAGCAGTATAATGCACTGCTGAGCAATATGAGTCGTATCTATAGTACCGCAAAAGTGTGCCTGCCGAATAAAACCGCAACCTGTTGGAGTCTGGATCCGGATCTGACCAATATTCTGGCAAGTAGCCGCAGTTATGCCATGCTGCTGTTCGCCTGGGAAGGTTGGCATAATGCAGCCGGCATTCCGCTGAAACCGCTGTATGAAGACTTCACCGCACTGAGCAATGAAGCATATAAACAGGATGGCTTCACCGATACCGGTGCATATTGGCGTAGTTGGTATAATAGCCCGACCTTCGAAGATGATCTGGAACATCTGTATCAGCAGCTGGAACCGCTGTATCTGAATCTGCATGCATTCGTGCGTCGCGCCCTGCATCGCCGTTATGGTGATCGTTATATTAATCTGCGCGGTCCGATTCCGGCACATCTGCTGGGCGATATGTGGGCCCAGAGCTGGGAAAATATCTATGATATGGTGGTGCCGTTCCCGGATAAACCGAATCTGGATGTGACCAGTACCATGCTGCAGCAGGGTTGGAATGCCACCCACATGTTCCGTGTTGCAGAAGAATTCTTCACCAGCCTGGAACTGAGTCCGATGCCGCCGGAATTCTGGGAAGGTAGCATGCTGGAAAAACCGGCCGATGGTCGTGAAGTTGTGTGCCATGCAAGTGCATGGGACTTCTATAATCGCAAAGACTTCCGCATTAAACAGTGTACCCGCGTGACCATGGATCAGCTGAGCACCGTGCATCATGAAATGGGCCATATTCAGTATTATCTGCAGTATAAAGACCTGCCGGTGAGCCTGCGCCGTGGTGCAAATCCGGGCTTCCATGAAGCCATTGGCGATGTTCTGGCACTGAGCGTTAGCACCCCGGAACATCTGCATAAAATTGGTCTGCTGGATCGTGTGACCAATGATACCGAAAGCGATATTAATTATCTGCTGAAAATGGCACTGGAAAAAATTGCATTCCTGCCGTTCGGTTATCTGGTTGATCAGTGGCGCTGGGGTGTGTTCAGCGGTCGTACCCCGCCGAGCCGTTATAACTTCGATTGGTGGTATCTGCGTACCAAATATCAGGGTATCTGTCCGCCGGTTACCCGCAATGAAACACACTTCGATGCCGGTGCCAAATTCCATGTTCCGAATGTGACCCCGTATATTCGTTACTTCGTGAGCTTCGTGCTGCAGTTCCAGTTCCATGAAGCACTGTGCAAAGAAGCCGGCTATGAAGGTCCGCTGCATCAGTGTGATATCTATCGTAGTACCAAAGCCGGTGCAAAACTGCGTAAAGTGCTGCAGGCCGGCAGTAGCCGTCCGTGGCAGGAAGTTCTGAAAGATATGGTGGGTCTGGATGCACTGGATGCCCAGCCGCTGCTGAAATACTTCCAGCCGGTTACCCAGTGGCTGCAGGAACAGAATCAGCAGAATGGCGAAGTTCTGGGCTGGCCGGAATATCAGTGGCATCCG

Expression Vector: pet-22b(+)

Research Use Only