CD146 Polyclonal Antibody-BS90217

CD146 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90217-50, BS90217-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P43121(Human) Q8R2Y2(Mouse) Q9EPF2(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: The tumorigenic and metastatic phenotype of melanoma cells correlates well with an increased expression of cell-cell and cell-matrix adhesion receptors. The human Mel-CAM gene encodes a transmembrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. The deduced human sequence of 603 amino acids consists of a signal peptide, five immunoglobulin-like domains, a transmembrane region and a short cytoplasmic tail. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human mela-nomas and correlates well with development of metastatic disease. Mel-CAM activation initiates an outside-in signaling pathway that involves the protein tyrosine kinases Fyn, FAK and paxillin. Mel-CAM influences the dynamics of Actin cytoskeleton rearrangement and is essential for the maintenance of thymic architecture and function.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD146 polyclonal antibody detects endogenous levels of CD146 protein.

Bioworld Molecular Weight: 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD146 on SiHa cell using anti-CD146 antibody at 1/500 dilution., ICC staining CD146 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MCAM, CD146, MUC18, A32 antigen, CD 146, CD146, CD146 antigen, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Gicerin, Mcam, Melanoma adhesion molecule, Melanoma associated antigen A32, Melanoma associated antigen MUC18, Melanoma associated glycoprotein MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MelCAM, MUC 18, MUC18, MUC18, S endo 1, S endo 1 endothelial associated antigen, S-endo 1 endothelial-associated antigen,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified