Product Description

CD146 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0270P-50, NCP0270P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P43121

Host: Rabbit

Reactivity: Human

Applications: WB, IP, IF

All Applications: WB: 1:2000~1:5000
IF 1:50~1:200
IP 1:50 - 1:200

Background: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD146 polyclonal antibody detects endogenous levels of CD146 protein.

Bioworld Molecular Weight: ~110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD146 polyclonal antibody at 1:2000 dilution
Lane 1: L02 cell membrane lysate (20ug)
Lane 2: A549 cell membrane lysate (20ug)
Lane 3: MCF-7 cell membrane lysate (20ug), Immunofluorescence analysis of L02 cells using CD146 pAb at dilution of 1:200., Immunoprecipitation of MCF-7 cell lysates using CD146 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CD146 pAb #NCP0270P.

Alternative Name: Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146

Immunogen: Recombinant protein of human CD146.

Conjugate: Unconjugated

Modification: Unmodified