Product Description

CD146 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0270-500, NCP0270-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Melanoma cell adhesion molecμle (MCAM, MUC18, CD146) is an immunoglobμlin superfamily member originally described as a cell surface adhesion protein and marker of the progression and metastasis of melanoma. Expression of MCAM protein is seen in vascμlar endothelial cells, activated T lymphocytes, smooth muscle, and bone marrow stromal cells. Research studies demonstrate increased MCAM expression in endothelial cells from angiogenesis-related disorders, including inflammatory bowel disease, Crohn’s disease, rheumatoid arthritis, tumors, and chronic renal failure. MCAM-expressing human mesenchymal stromal cells (hMSC) in the hematopoietic microenvironment are responsible for maintaining the self-renewal of hematopoietic stem and progenitor cells (HSPC) through direct contact between hMSC and HSPC. Related studies suggest that activation of the Notch signaling pathway may also, in part, play a role in HSPC maintenance. Additional research indicates that MCAM may play a role in mμltiple sclerosis, an autoimmune inflammatory disease that affects central nervous system neurons. Endothelial MCAM within the blood-brain barrier act as adhesion receptors that permit lymphocytes to transmigrate across the barrier and produce the inflammatory lesions that characterize the disorder.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~59kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P43121

Tag: His-tag

Amino Acid Sequence: GTTCCGGGTGAAGCAGAACAGCCGGCACCGGAACTGGTGGAAGTTGAAGTGGGTAGTACCGCACTGCTGAAATGCGGTCTGAGCCAGAGTCAGGGTAATCTGAGCCATGTGGATTGGTTCAGCGTTCATAAAGAAAAACGTACCCTGATCTTCCGTGTGCGTCAGGGCCAGGGTCAGAGCGAACCGGGTGAATATGAACAGCGTCTGAGTCTGCAGGATCGTGGCGCAACCCTGGCCCTGACCCAGGTGACCCCGCAGGATGAACGCATCTTCCTGTGCCAGGGCAAACGCCCGCGCAGTCAGGAATATCGCATTCAGCTGCGCGTGTATAAAGCCCCGGAAGAACCGAATATTCAGGTTAATCCGCTGGGTATTCCGGTGAATAGCAAAGAACCGGAAGAAGTGGCAACCTGCGTTGGCCGCAATGGCTATCCGATTCCGCAGGTTATCTGGTATAAAAATGGTCGTCCGCTGAAAGAAGAAAAAAATCGCGTGCATATTCAGAGCAGCCAGACCGTTGAAAGTAGCGGCCTGTATACCCTGCAGAGCATTCTGAAAGCACAGCTGGTTAAAGAAGATAAAGATGCCCAGTTCTATTGCGAACTGAATTATCGCCTGCCGAGTGGCAATCACATGAAAGAAAGCCGCGAAGTGACCGTTCCGGTGTTCTATCCGACCGAAAAAGTGTGGCTGGAAGTTGAACCGGTTGGCATGCTGAAAGAAGGTGATCGTGTTGAAATTCGTTGCCTGGCCGATGGCAATCCGCCGCCGCACTTCAGCATTAGTAAACAGAATCCGAGCACCCGTGAAGCAGAAGAAGAAACCACCAATGATAATGGTGTGCTGGTGCTGGAACCGGCCCGCAAAGAACATAGTGGCCGTTATGAATGCCAGGGCCTGGATCTGGATACCATGATTAGTCTGCTGAGCGAACCGCAGGAACTGCTGGTTAATTATGTTAGCGATGTTCGCGTTAGTCCGGCCGCCCCGGAACGTCAGGAAGGTAGCAGTCTGACCCTGACCTGTGAAGCCGAAAGTAGTCAGGATCTGGAATTCCAGTGGCTGCGTGAAGAAACCGGTCAGGTTCTGGAACGCGGCCCGGTGCTGCAGCTGCATGATCTGAAACGTGAAGCAGGTGGTGGTTATCGTTGTGTGGCAAGTGTTCCGAGCATTCCGGGCCTGAATCGTACCCAGCTGGTGAATGTTGCCATCTTCGGCCCGCCGTGGATGGCATTCAAAGAACGTAAAGTGTGGGTTAAAGAAAATATGGTGCTGAATCTGAGTTGTGAAGCAAGTGGCCATCCGCGCCCGACCATTAGCTGGAATGTTAATGGTACCGCAAGCGAACAGGATCAGGATCCGCAGCGTGTGCTGAGCACCCTGAATGTGCTGGTTACCCCGGAACTGCTGGAAACCGGCGTTGAATGTACCGCAAGCAATGATCTGGGTAAAAATACCAGTATTCTGTTCCTGGAACTGGTGAATCTGACCACCCTGACCCCGGATAGTAATACCACCACCGGCCTGAGCACCAGCACCGCCAGCCCTCATACCCGTGCAAATAGTACCAGTACCGAACGCAAACTGCCGGAACCGGAAAGTCGTGGC

Expression Vector: pet-22b(+)

Research Use Only