Product Description

CD147 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0177P-50, NCP0177P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P35613

Host: Rabbit

Reactivity: Human

Applications: WB, IP,

All Applications: WB: 1:2000~1:5000
IP 1:50 - 1:200

Background: Basigin (EMMPRIN, CD147) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. Basigin is a glycosylated protein with four known isoforms, of which isoform 2 is the most abundantly expressed. Multiple functions have been ascribed to Basigin; foremost among these is stimulating the secretion of extracellular matrix metalloproteinases by adjacent fibroblasts, a function which has been implicated in promoting tumor progression. Research studies have shown that Basigin is overexpressed by many tumor cells, and its expression level may correlate with tumor malignancy. A recent study identified the BASIGIN gene as a regulatory target of Slug, suggesting a role for Basigin in the process of epithelial-mesenchymal transition. Basigin has also been identified as a marker for a subset of highly suppressive regulatory T cells, and as an obligate receptor for the malarial parasite Plasmodium falciparum on human erythrocytes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD147 polyclonal antibody detects endogenous levels of CD147 protein.

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD147 polyclonal antibody at 1:5000 dilution
Lane 1: A375 cell membrane lysate (10ug)
Lane 2: SK-OVCAR3 cell membrane lysate (4ug), Western blot (WB) analysis of CD147 polyclonal antibody at 1:2000 dilution
Lane 1: EC9706 cell membrane lysate (25ug)
Lane 2: A549 cell membrane lysate (25ug), Immunoprecipitation of SK-OVCAR3 cell lysates using CD147 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CD147 pAb #NCP0177P.

Alternative Name: Basigin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Hepatoma-associated antigen; HAb18G; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD147; BSG; UNQ6505/PRO21383

Immunogen: Recombinant protein of human CD147.

Conjugate: Unconjugated

Modification: Unmodified