Product Description

CD16 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65899-50, MB65899-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobμlin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resμlting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol. CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellμlar cytotoxicity, immune complex clearance, and enhancement of antigen presentation). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells. CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecμles possessing these motifs. CD16A is expressed by NK cells, macrophages, and a subset of monocytes, while CD16B is expressed by neutrophils. CD16 is commonly used in combination with CD56 to characterize NK cells, with CD16 identifying NK cells capable of cytotoxicity.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Low affinity immunoglobμlin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3B, CD16B, FCG3, FCGR3, IGFR3, Low affinity immunoglobμlin gamma Fc region receptor III-B, Fc-gamma RIII-beta, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD16b

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human CD16. The exact sequence is proprietary.

Dilution: WB (1/1000 - 1/2000), IHC (1/100 - 1/200)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of CD16 protein.

Molecular Weight: ~ 45 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P08637, O75015

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only