CD163 Recombinant Protein - NCP0287

CD163 Recombinant Protein

Catalogue Number: NCP0287-500, NCP0287-1000

Sizes: 500ug, 1mg

Swiss-Prot: Q86VB7

Host: E.coli

Tag: His-tag

AA Sequence: AGCAGCCTGGGCGGTACCGATAAAGAACTGCGTCTGGTGGATGGTGAAAATAAATGCAGTGGTCGTGTGGAAGTGAAAGTTCAGGAAGAATGGGGCACCGTGTGTAATAATGGTTGGAGCATGGAAGCCGTTAGCGTGATCTGTAATCAGCTGGGCTGCCCGACCGCAATTAAAGCCCCGGGCTGGGCCAATAGTAGTGCAGGCAGTGGCCGTATCTGGATGGATCATGTTAGCTGCCGTGGCAATGAAAGCGCCCTGTGGGATTGTAAACATGATGGTTGGGGCAAACATAGTAATTGTACCCATCAGCAGGATGCCGGTGTTACCTGTAGTGATGGCAGTAATCTGGAAATGCGTCTGACCCGCGGTGGCAATATGTGCAGTGGCCGTATTGAAATTAAATTCCAGGGCCGCTGGGGCACCGTGTGTGATGATAACTTCAATATTGATCATGCAAGTGTTATCTGTCGTCAGCTGGAATGTGGTAGCGCCGTTAGCTTCAGTGGCAGTAGTAACTTCGGTGAAGGCAGTGGTCCGATCTGGTTCGATGATCTGATCTGTAATGGTAATGAAAGTGCCCTGTGGAATTGTAAACATCAGGGTTGGGGTAAACATAATTGTGATCATGCCGAAGATGCCGGTGTGATCTGTAGTAAAGGCGCAGATCTGAGTCTGCGTCTGGTTGATGGTGTGACCGAATGCAGTGGTCGCCTGGAAGTTCGCTTCCAGGGTGAATGGGGTACCATCTGTGATGATGGTTGGGATAGCTATGATGCAGCAGTTGCCTGTAAACAGCTGGGCTGTCCGACCGCAGTGACCGCCATTGGTCGCGTGAATGCAAGTAAAGGCTTCGGTCATATCTGGCTGGATAGCGTTAGCTGCCAGGGCCATGAACCGGCAATCTGGCAGTGCAAACATCATGAATGGGGCAAACACTATTGTAATCATAATGAAGATGCCGGCGTGACCTGTAGTGACGGTAGTGATCTGGAACTGCGTCTTCGCGGTGGTGGCAGTCGTTGTGCAGGTACCGTTGAAGTTGAAATTCAGCGTCTGCTGGGTAAAGTGTGTGATCGCGGCTGGGGTCTGAAAGAAGCAGATGTTGTGTGCCGCCAGCTGGGTTGTGGCAGCGCCCTGAAAACCAGTTATCAGGTGTATAGTAAAATCCAGGCAACCAATACCTGGCTGTTCCTGAGCAGTTGTAATGGTAACGAAACCAGTCTGTGGGATTGCAAAAATTGGCAGTGGGGTGGCCTGACCTGTGATCATTATGAAGAAGCCAAAATTACCTGTAGCGCACATCGCGAACCGCGCCTGGTTGGCGGCGATATTCCGTGCAGTGGCCGCGTTGAAGTTAAACATGGTGATACCTGGGGCAGTATCTGTGATAGCGACTTCAGTCTGGAAGCAGCCAGCGTTCTGTGTCGCGAACTGCAGTGTGGCACCGTTGTTAGTATTCTGGGCGGCGCACACTTCGGCGAAGGCAATGGTCAGATCTGGGCAGAAGAATTCCAGTGTGAAGGTCATGAAAGCCATCTGAGTCTGTGTCCGGTTGCACCGCGTCCGGAAGGCACCTGCAGCCATAGTCGCGATGTTGGTGTTGTGTGTAGCCGCTATACCGAAATTCGTCTGGTTAATGGCAAAACACCTTGCGAAGGTCGTGTTGAACTGAAAACCTTAGGTGCCTGGGGTAGCCTGTGCAATAGTCATTGGGATATTGAAGATGCCCATGTTCTGTGCCAGCAGCTGAAATGCGGTGTTGCACTGAGTACCCCGGGCGGCGCACGCTTCGGTAAAGGTAATGGCCAGATCTGGCGTCACATGTTCCATTGCACCGGCACCGAACAGCACATGGGCGATTGTCCGGTTACCGCCCTGGGTGCCAGCCTGTGTCCGAGCGAACAGGTGGCAAGCGTGATCTGTAGCGGCAATCAGAGTCAGACCCTGAGCAGCTGCAATAGTAGCAGCCTGGGTCCGACCCGTCCGACCATTCCGGAAGAAAGCGCCGTGGCCTGCATTGAAAGCGGTCAGCTGCGTCTGGTCAATGGTGGCGGTCGTTGTGCCGGTCGTGTGGAGATCTATCATGAAGGCAGTTGGGGTACCATCTGTGACGATAGTTGGGATCTGAGCGATGCCCATGTGGTGTGTCGTCAGTTAGGTTGCGGCGAAGCCATTAATGCAACCGGTAGCGCACACTTCGGTGAAGGTACCGGTCCGATCTGGCTGGACGAAATGAAATGCAATGGTAAAGAAAGCCGTATCTGGCAGTGTCATAGCCATGGTTGGGGCCAGCAGAATTGCCGCCATAAAGAAGATGCAGGTGTTATCTGTAGCGAATTCATGAGCCTGCGCCTGACCAGTGAAGCCAGTCGCGAAGCATGCGCCGGCCGTCTGGAAGTGTTCTATAATGGTGCCTGGGGCACCGTT

Restriction Sites: NdeI-XhoI

Background: CD163 is a transmembrane scavenger receptor expressed on the macrophage surface. It has 9 B-type SRCR extracellular domains mediating serum haptoglobin clearing/endocytosis, pathogen binding and signal transduction, and calcium binding. CD163 is used as a surface marker of M2 type macrophages, including M2 type tumor associated macrophages (TAMs), which facilitate cancer progression by secreting cytokines to promote angiogenesis, immunosuppression and metastasis. Inflammatory stimulation and stress signal can induce extracellular domain shedding of CD163 to generate soluble CD163 (sCD163). The increased sCD163 level in serum is associated with low-grade inflammation in disease conditions.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~89kDa

Notes: For research use only, not for use in diagnostic procedure.