Product Description

CD169 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0291-500, NCP0291-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granμles of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A (Chr-A); chromogranin B (Chr-B), also known as secretogranin I; chromogranin C (also known as secretogranin II or Sg II); and secretogranin III (Sg III or SCG3). High levels of Chr-A expression are characteristic of neuroendocrine tumors. Pancreastatin is a peptide derived from Chr-A which inhibits Insμlin secretion, exocrine pancreatic secretion and gastric acid secretion. Pancreastatin exists as two forms; the major form is expressed in stomach and colon extracts. In neuroendocrine cells the level Sg II has been shown to increase four-fold in response to histamine, while levels of Chr-A and Chr-B showed little or no increase. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterolrich secretory granμle (SG) membranes.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~86kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: Q9BZZ2

Tag: His-tag

Amino Acid Sequence: AGCTGGGGTGTGAGCAGCCCGCAGGATGTGCAGGGCGTTAAAGGTAGTTGCCTGCTGATTCCGTGTATCTTCAGCTTCCCGGCAGATGTTGAAGTGCCGGATGGTATTACCGCAATCTGGTATTATGATTATAGCGGTCAGCGTCAGGTGGTGAGCCATAGCGCCGATCCGAAACTGGTGGAAGCCCGCTTCCGCGGCCGCACAGAATTCATGGGTAATCCGGAACATCGTGTGTGCAATCTGCTGCTGAAAGATCTGCAGCCGGAAGATAGCGGTAGTTATAACTTCCGCTTCGAAATTAGTGAAGTTAATCGCTGGAGTGATGTTAAAGGCACCCTGGTGACCGTTACCGAAGAACCGCGTGTGCCGACCATTGCCAGTCCGGTTGAACTGCTGGAAGGCACCGAAGTTGACTTCAATTGCAGTACCCCGTATGTGTGCCTGCAGGAACAGGTTCGCCTGCAGTGGCAGGGTCAGGATCCGGCCCGCAGTGTTACCTTCAATAGTCAGAAATTCGAACCGACCGGTGTGGGTCATCTGGAAACCTTACACATGGCCATGAGTTGGCAGGATCATGGCCGCATTCTGCGCTGTCAGCTGAGTGTTGCAAATCATCGCGCACAGAGTGAAATTCATCTGCAGGTTAAATATGCCCCGAAAGGTGTGAAAATTCTGCTGAGCCCGAGTGGTCGCAATATTCTGCCGGGCGAACTGGTTACCCTGACCTGCCAGGTGAATAGCAGCTATCCGGCCGTTAGCAGTATTAAATGGCTGAAAGATGGCGTTCGTCTGCAGACCAAAACCGGTGTTCTGCATCTGCCGCAGGCAGCCTGGAGTGATGCAGGTGTGTATACCTGTCAGGCCGAAAATGGCGTGGGTAGTCTGGTGAGCCCGCCGATTAGTCTGCATATCTTCATGGCCGAAGTGCAGGTGAGCCCGGCCGGTCCTATTCTGGAAAATCAGACCGTGACCCTGGTGTGTAATACCCCGAATGAAGCCCCGAGCGATCTGCGCTATAGCTGGTATAAAAATCATGTTCTGCTGGAAGATGCCCATAGCCATACCCTGCGCCTGCATCTGGCAACCCGCGCAGATACCGGCTTCTACTTCTGCGAAGTTCAGAATGTGCATGGCAGCGAACGCAGTGGCCCGGTTAGTGTTGTTGTGAATCATCCGCCGCTGACCCCGGTGCTGACCGCATTCCTGGAAACACAAGCAGGTCTGGTGGGCATTCTGCATTGTAGCGTGGTGAGCGAACCGCTGGCAACCCTGGTGCTGAGCCATGGTGGTCATATTCTGGCAAGCACCAGTGGCGATAGCGATCATAGTCCGCGCTTCAGTGGTACCAGCGGTCCGAATAGTCTGCGTCTGGAAATTCGTGATCTGGAAGAAACCGATAGCGGTGAATATAAATGTAGTGCAACCAATAGCCTGGGCAATGCCACCAGTACCCTGGACTTCCATGCCAATGCAGCCCGCCTGCTGATTAGTCCGGCAGCAGAAGTTGTTGAAGGCCAGGCAGTTACCCTGAGCTGCCGCAGTGGCCTGAGTCCGACCCCGGATGCCCGCTTCAGCTGGTATCTGAATGGTGCACTGCTGCATGAAGGTCCGGGTAGTAGTCTGCTGCTGCCGGCAGCAAGCAGCACCGATGCCGGTAGTTATCATTGCCGCGCACGCGATGGCCATAGTGCCAGCGGCCCGAGCAGTCCGGCCGTTCTTACCGTTCTGTATCCGCCGCGTCAGCCGACCTTCACCACCCGTCTGGATCTGGATGCCGCCGGCGCCGGTGCTGGTAGACGTGGTCTGCTGCTGTGCCGCGTTGATAGTGATCCGCCGGCACGTCTGCAGCTGCTGCATAAAGATCGCGTTGTTGCCACCAGTCTGCCGAGTGGCGGTGGTTGCAGTACCTGTGGTGGTTGTAGCCCGCGTATGAAAGTGACCAAAGCACCGAATCTGCTGCGTGTTGAAATTCATAATCCGCTGCTGGAAGAAGAAGGTCTGTATCTGTGCGAAGCAAGTAATGCACTGGGCAATGCGAGCACCAGCGCAACCTTCAATGGTCAGGCCACCGTGCTGGCAATTGCCCCGAGCCATACCTTACAGGAAGGTACCGAAGCCAATCTGACCTGCAATGTTAGTCGCGAAGCAGCAGGTAGTCCGGCAAACTTCAGTTGGTTCCGCAATGGTGTTCTGTGGGCACAGGGCCCGCTGGAAACCGTGACCTTACTGCCGGTGGCCCGTACCGATGCCGCCTTATATGCCTGTCGCATTCTGACCGAAGCAGGTGCCCAGCTGAGTACCCCGGTGTTACTGAGTGTGCTGTATCCGCCTGATCGCCCGAAA

Expression Vector: pet-22b(+)

Research Use Only