Product Description

CD171/N-CAML1 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0182-500, NCP0182-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Neural cell adhesion molecμle L1 (NCAM-L1/L1CAM) is a single pass transmembrane glycoprotein member of the immunoglobμlin superfamily, containing six amino-terminal extracellμlar Ig-like domains followed by five fibronectin type-III domains. NCAM-L1 is mainly expressed in the brain, and plays an important role in the developing nervous system, with involvement in neurite fascicμlation and outgrowth, myelination, neuronal migration, and neuronal cell adhesion. Mutations in the NCAM-L1 gene cause varying degrees of neurological disease including X-linked hydrocephalus, MASA syndrome, spastic paraplegia type 1, and X-linked corpus callosum agenesis, together known as L1 syndrome. Apart from the nervous system, NCAM-L1 is overexpressed in many cancers and supports a poor prognosis by facilitating aggressive tumor growth, metastasis and chemoresistance.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~36kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P32004

Tag: His-tag

Amino Acid Sequence: CAGGGTAAAGGCCCGGAACCGCAGGTGACCATTGGTTATAGCGGTGAAGATTATCCGCAGGCAATTCCGGAACTGGAAGGTATTGAAATTCTGAATAGCAGTGCCGTGCTGGTTAAATGGCGCCCGGTGGATCTGGCCCAGGTGAAAGGTCATCTGCGCGGTTATAATGTTACCTATTGGCGTGAAGGTAGCCAGCGTAAACATAGCAAACGTCATATTCATAAAGACCATGTGGTTGTGCCGGCCAATACCACCAGCGTGATTCTGAGCGGTCTGCGTCCGTATAGCAGTTATCATCTGGAAGTTCAGGCCTTCAATGGCCGCGGCAGCGGCCCTGCTAGTGAATTCACCTTCAGCACCCCGGAAGGTGTTCCGGGCCATCCGGAAGCACTGCATCTGGAATGCCAGAGTAATACCAGTCTGCTGCTGCGTTGGCAGCCGCCGCTGAGTCATAATGGCGTTCTGACCGGTTATGTGCTGAGCTATCATCCGCTGGATGAAGGTGGCAAAGGTCAGCTGAGCTTCAATCTGCGCGATCCGGAACTGCGTACCCATAATCTGACCGATCTGAGTCCGCATCTGCGCTATCGCTTCCAGCTGCAGGCAACCACCAAAGAAGGCCCGGGTGAAGCCATTGTGCGCGAAGGTGGTACCATGGCACTGAGTGGTATTAGTGACTTCGGCAATATTAGTGCCACCGCAGGTGAAAATTATAGTGTTGTGAGTTGGGTGCCGAAAGAAGGTCAGTGTAACTTCCGCTTCCATATTCTGTTCAAAGCACTGGGCGAAGAAAAAGGCGGCGCCAGCCTGAGTCCGCAGTATGTGAGTTATAATCAGAGTAGTTATACCCAGTGGGATCTGCAGCCGGATACCGATTATGAAATTCATCTGTTCAAAGAACGCATGTTCCGCCATCAGATGGCCGTGAAAACCAATGGCACCGGTCGTGTTCGTCTGCCGCCGGCAGGCTTCGCAACCGAA

Expression Vector: pet-22b(+)

Research Use Only