Product Description

CD172g Polyclonal Antibody

Catalogue Numbers: BS62483-50, BS62483-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9P1W8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domaincontaining protein-tyrosine phosphatase SHP-2 in response to Insulin. The SIRP family negatively regulates the PI 3-K pathway, which may diminish EGFR-mediated motility and survival phenotypes that contribute to transformation of certain cell types. SIRP-α1 is a transmembrane protein which contains an extracellular portion with three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. SIRP-α1 is a substrate for activated receptor tyrosine kinases. In its tyrosine phosphorylated form, SIRP-α1 binds to SH-PTP2 through SH2 interactions and acts as an SH-PTP2 substrate. SIRP-α1 has been shown to have negative regulatory effects on cellular responses induced by growth factors, oncogenes and insulin. SIRP-β1 shares extensive sequence homology with SIRP-α1 in its extracellular portion but lacks the cytoplasmic portion. SIRP-γ, originally designated SIRP-β2 (SIRP-B2, CD172g) has unique characteristics from both the α and β versions. SIRP-γ is expressed on the majority of T cells and a proportion of B cells. CD47 associates with SIRP-γ, and this interaction signals unidirectionally only.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD172g polyclonal antibody detects endogenous levels of CD172g protein.

Extra Notes: Western blot (WB) analysis of CD172g polyclonal antibody at 1:500 dilution
LaneA:AML-12 whole cell lysate
LaneB:PC12 whole cell lysate
LaneC:L02 whole cell lysate
LaneD:HEK293T whole cell lysate

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Signal-regulatory protein gamma; SIRP-gamma; CD172 antigen-like family member B; Signal-regulatory protein beta-2; SIRP-b2; SIRP-beta-2; CD172g; SIRPG; SIRPB2

Immunogen: Synthetic peptide, corresponding to Human CD172g.

Conjugate: Unconjugated

Modification: Unmodified