CD178 Polyclonal Antibody-NCP0183P

CD178 Polyclonal Antibody

Catalogue Numbers: NCP0183P-50, NCP0183P-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P48023

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers. Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes. FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8. Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites. FasL is also expressed in several tumor types as a mechanism to evade immune surveillance. Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD178 polyclonal antibody detects endogenous levels of CD178 protein.

Extra Notes: Western blot (WB) analysis of CD178 polyclonal antibody at 1:1000 dilution
Lane1:L02 cell membrane lysate(24ug)
Lane2:EC9706 cell membrane lysate(24ug)

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Tumor necrosis factor ligand superfamily member 6; Apoptosis antigen ligand; APTL; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD178; Receptor-binding FasL ectodomain; Soluble Fas ligand; sFasL; ADAM10-processed FasL form; APL; FasL ICD; SPPL2A-processed FasL form; SPA; FASLG; APT1LG1; CD95L; FASL; TNFSF6

Immunogen: Recombinant protein of human CD178.

Conjugate: Unconjugated

Modification: Unmodified