Product Description

CD180 Recombinant Protein

Catalogue Number: NCP0184-500, NCP0184-1000

Sizes: 500ug, 1mg

Swiss-Prot: Q99467

Host: E.coli

Tag: His-tag

AA Sequence: TGGGATCAGATGTGCATTGAAAAAGAAGCCAATAAAACCTATAACTGTGAAAATCTGGGCCTGAGTGAAATTCCGGATACCCTGCCGAATACCACCGAATTCCTGGAATTCAGCTTCAACTTCCTGCCGACCATTCATAATCGCACCTTCAGCCGCCTGATGAATCTGACCTTCCTGGATCTGACCCGCTGTCAGATTAATTGGATTCATGAAGATACCTTCCAGAGCCATCATCAGCTGAGCACCCTGGTTCTGACCGGTAATCCGCTGATCTTCATGGCCGAAACCAGTCTGAATGGTCCGAAAAGCCTGAAACATCTGTTCCTGATTCAGACCGGTATTAGCAATCTGGAATTCATTCCGGTGCATAATCTGGAAAATCTGGAAAGCCTGTATCTGGGCAGTAATCATATTAGTAGCATTAAATTCCCGAAGGACTTCCCGGCCCGTAATCTGAAAGTTCTGGACTTCCAGAATAATGCAATTCATTATATCAGCCGCGAAGATATGCGTAGTCTGGAACAGGCAATTAATCTGAGCCTGAACTTCAATGGCAATAATGTGAAAGGCATTGAACTGGGTGCCTTCGATAGTACCATCTTCCAGAGTCTGAACTTCGGTGGTACCCCGAATCTGAGCGTTATCTTCAATGGTCTGCAGAATAGCACCACCCAGAGCCTGTGGCTGGGCACCTTCGAAGATATTGATGATGAAGATATTAGCAGTGCCATGCTGAAAGGTCTGTGCGAAATGAGCGTTGAAAGTCTGAATCTGCAGGAACATCGCTTCAGTGATATTAGCTCAACCACCTTCCAGTGCTTCACCCAGCTGCAGGAACTGGATCTGACAGCAACCCATCTGAAAGGCCTGCCGAGTGGCATGAAAGGCCTGAATCTGCTGAAAAAACTGGTGCTGAGCGTTAATCACTTCGATCAGCTGTGCCAGATTAGCGCAGCAAACTTCCCGAGCCTGACCCATCTGTATATTCGCGGTAATGTGAAAAAACTGCATCTGGGCGTTGGTTGTCTGGAAAAACTGGGTAATCTGCAGACCCTGGATCTGAGCCATAATGATATTGAAGCAAGCGATTGTTGTAGCCTGCAGCTGAAAAATCTGAGCCATCTGCAGACCTTAAATCTGAGTCATAATGAACCGCTGGGTCTGCAGAGTCAGGCATTCAAAGAATGTCCGCAGCTGGAACTGCTGGATCTGGCCTTCACCCGCCTGCATATTAATGCACCGCAGAGCCCGTTCCAGAATCTGCACTTCCTGCAGGTGCTGAATCTGACCTATTGCTTCCTGGATACCAGCAATCAGCATCTGCTGGCCGGCCTGCCGGTTCTGCGTCATCTGAATCTGAAAGGCAATCACTTCCAGGATGGTACCATTACCAAAACCAATCTGCTGCAGACCGTGGGCAGCCTGGAAGTGCTGATTCTGAGCAGCTGCGGCCTGCTGAGTATTGATCAGCAGGCCTTCCATAGCCTGGGCAAAATGAGTCATGTTGATCTGAGCCACAATAGCCTGACCTGTGATAGCATTGATAGCCTGAGCCATCTTAAAGGTATCTATCTGAATCTGGCCGCCAATAGTATTAATATTATTAGCCCGCGTCTGCTGCCGATTCTGAGTCAGCAGAGCACCATTAATCTGTCACATAATCCGCTGGATTGCACCTGCAGTAATATTCACTTCCTGACCTGGTATAAAGAAAATCTGCATAAACTGGAAGGTAGCGAAGAAACCACCTGTGCAAATCCGCCGAGCCTGCGCGGTGTTAAACTGAGCGATGTTAAACTGAGTTGTGGC

Restriction Sites: NdeI-XhoI

Background: CD180/RP105 is a type-I transmembrane glycoprotein that is restricted to expression in mature B-cells. Research studies have shown that CD180 engages signaling cascades in mature B-cells that drive cell proliferation. Indeed, CD180 functions with TLR4 as an LPS receptor on B-cells and, upon binding LPS, transmits proliferation and survival signals that require the Vav family of guanine nucleotide exchange factors.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~66kDa

Notes: For research use only, not for use in diagnostic procedure.