CD1E Polyclonal Antibody
Catalogue Numbers: BS5652-50, BS5652-100
Sizes: 50µl, 100µl
Alternative Name: T-cell surface glycoprotein CD1e, membrane-associated; hCD1e; R2G1; CD1e; T-cell surface glycoprotein CD1e, soluble; sCD1e
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P15812
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB ICC
All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200
Background: CD1E, also known as R2 or CD1A, is a 322 amino acid single-pass type I membrane protein that localizes to the lumen of the endoplasmic reticulum, as well as to the golgi apparatus, and contains one Ig-like domain. Expressed in a variety of tissues and on cortical thymocytes, dendritic cells and Langerhans cells, CD1E exists as a heterodimer with β-2-Microglobulin and is necessary for the presentation of glycolipid antigens on the cell surface. CD1E is subject to post-translational mono-ubiquitination and may also be proteolytically cleaved in endosomes to yield a soluble protein. CD1E is present on the surface of some T-cell leukemias, suggesting a possible role in tumorigenesis.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: CD1E polyclonal antibody detects endogenous levels of CD1E protein.
Bioworld Molecular Weight: ~ 44 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of CD1E polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate
Immunogen: Synthetic peptide, corresponding to amino acids 258-303 of Human CD1E.
Conjugate: Unconjugated
Modification: Unmodified