CD20 Monoclonal Antibody-MB65762

CD20 Monoclonal Antibody

Catalogue Numbers: MB65762-50, MB65762-100

Sizes: 50µl, 100µl

Product: Mouse IgG2a. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P11836

Host: Mouse

Reactivity: Human

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: B-lymphocyte antigen CD20 (also known as MS4A1; Membrane-spanning 4-domains subfamily A member 1) is a cell surface phosphoprotein involved in the regulation of B cell activation and proliferation. It is commonly used as a marker to identify B cells and is expressed throughout B cell development, up until their differentiation into plasma cells. CD20 has no known ligand, and its expression and function are largely conserved between human and mouse. Evidence suggests that CD20 is necessary for store operated calcium (SOC) entry, which leads to elevated cytoplasmic calcium levels required for B cell activation . Anti-CD20 antibody immunotherapy depletes B cells by activation of the innate monocytic network and is a common treatment for B cell lymphomas, leukemias, and autoimmune diseases.

Purification and Purity: The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes human CD20

Extra Notes: Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD20 Antibody, followed by anti-mouse IgG PE.

Bioworld Molecular Weight: ~ 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20

Immunogen: Native purified human CD20.

Conjugate: Unconjugated

Modification: Unmodified