Product Description

CD201 Recombinant Protein

Catalogue Number: NCP0349-500, NCP0349-1000

Sizes: 500ug, 1mg

Swiss-Prot: Q9UNN8

Host: E.coli

Tag: His-tag

AA Sequence: MSQDASDGLQRLHMLQISYFRDPYHVWYQGNASLGGHLTHVLEGPDTNTTIIQLQPLQEPESWARTQSGLQSYLLQFHGLVRLVHQERTLAFPLTIRCFLGCELPPEGSRAHVFFEVAVNGSSFVSFRPERALWQADTQVTSGVVTFTLQQLNAYNRTRYELREFLEDTCVQYVQKHISAENTKGSQTSRSYTS

Restriction Sites: NdeI-XhoI

Background: Thrombomodulin™ is an endothelial specific receptor that forms a complex with thrombin, a protein with procoagulant, inflammatory and anticoagulant effects. The TM/thrombin complex activates protein C (PC) to generate activated protein C (APC) and initiate the APC anticoagulant pathway. APC attenuates thrombin formation through the inactivation, by limited proteolysis, of two significant cofactors of blood clot formation, Factor Va and Factor VIIIa. This process is augmented by the activity of the endothelial cell Protein C receptor (EPCR), which binds both PC and APC with high affinity. The EPCR gene maps to human chromosome 20q11.22 and encodes an anticoagulant that is preferentially expressed on large blood vessel endothelium in the heart and lung with some expression in capillaries in the lung and skin. EPCR, also designated CCD41 in mouse, is a member of the major histocompatibility complex and displays significant homology to CD1. Soluble plasma EPCR is thought to inhibit the membrane-bound EPCR activation of the APC pathway.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~28kDa

Notes: For research use only, not for use in diagnostic procedure.