CD21 Monoclonal Antibody-MB65989

CD21 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB65989-50, MB65989-100

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: P20023

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: Complement receptor type 2 (CD21/CR2) is a type 1 transmembrane glycoprotein whose expression is largely restricted to B lymphocytes and follicular dendritic cells. Research studies have shown that CD21/CR2 functions to bind the complement fragments iC3b, C3dg, and C3d, which function to activate the alternative complement pathway and MAC formation. In addition to its function as a complement receptor, CD21/CR2 also functions as the B lymphocyte receptor for Epstein-Barr virus and interferon alpha. Research studies have also shown that CD21/CR2 participates in B cell activation, proliferation, and protection from apoptosis through its association with components of the B cell coreceptor signaling complex such as CD19 and CD21.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD21 protein.

Bioworld Molecular Weight: ~ 112 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of CD21 staining in human follicular lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of CD21 staining in human lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of CD21 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: C3DR; Complement receptor type 2; Cr2; Complement C3d receptor; Epstein-Barr virus receptor; EBV receptor; CD21

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CD21. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified