CD210 Polyclonal Antibody-NCP0331P

CD210 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0331P-50, NCP0331P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q13651

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IP, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200
IP 1:50 - 1:200

Background: Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD210 polyclonal antibody detects endogenous levels of CD210 protein.

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD210 polyclonal antibody at 1:1000 dilution
Lane 1: L02 whole cell lysate (30ug)
Lane 2: A549 whole cell lysate (30ug)
Lane 3: LOVO cell membrane lysate (24ug)
Lane 4: PC12 whole cell lysate (30ug), Immunofluorescence analysis of A549 cells using CD210 pAb at dilution of 1:200., Immunoprecipitation of MCF-7 cell lysates using CD210 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CD210 pAb #NCP0331P.

Alternative Name: Interleukin-10 receptor subunit alpha; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; CD_antigen: CD210; IL10RA; IL10R

Immunogen: Recombinant protein of human CD210.

Conjugate: Unconjugated

Modification: Unmodified